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Genes estructurales y reguladores requeridos para producir el gas dimetilsulfuro en bacterias
Jonathan D Todd1, Rachel Rogers, You Guo Li
1School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UK.
Science (New York, N.Y.)
|February 3, 2007
Resumen
Los científicos descubrieron que el gen bacteriano dddD es crucial para la producción de sulfuro de dimetilo (DMS) a partir de dimetilsulfoniopropionato (DMSP). Este hallazgo tiene un impacto en nuestra comprensión del ciclo global del azufre y la regulación del clima.
Área de la Ciencia:
- Microbiología Ambiental y Microbiología Ambiental.
- Ciclos biogeoquímicos y ciclos biogeoquímicos.
- Ciencias del clima Ciencias del clima Ciencias del clima Ciencias del clima
Sus antecedentes:
- El sulfuro de dimetilo (DMS) juega un papel vital en los ciclos globales del azufre y del carbono.
- Los productos de oxidación del DMS influyen en la formación de nubes, lo que puede afectar el clima y el clima.
- Las algas marinas producen dimetilsulfoniopropionato (DMSP), que las bacterias convierten en DMS.
Objetivo del estudio:
- Para identificar el gen bacteriano responsable de la producción de DMS a partir de DMSP.
- Para dilucidar el mecanismo enzimático de la liberación de DMS.
- Para investigar la regulación de la producción de DMS.
Principales métodos:
- Demostró el requisito del gen bacteriano dddD para la producción de DMS.
- Se ha demostrado que la transcripción de dddD es inducida por el sustrato DMSP.
- DddD clonado y expresado de diferentes especies bacterianas (Marinomonas, Rhizobium NGR234, Burkholderia cepacia AMMD) en Escherichia coli.
Principales resultados:
- El gen bacteriano dddD es esencial para la conversión de DMSP a DMS.
- La expresión de dddD clonado permitió la producción de DMS en E. coli.
- El mecanismo implica la modificación de la DMSP por la acilcoenzima A, no la actividad directa de la DMSP liasa.
Conclusiones:
- El gen dddD es un determinante clave en la producción bacteriana de DMS a partir de DMSP.
- Esta vía representa un nuevo mecanismo para la liberación del DMS.
- Comprender este proceso es fundamental para modelar el ciclo global del azufre y el clima.
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