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Estructura de la forma de prefusión de la glicoproteína G del virus de la estomatitis vesicular G
Stéphane Roche1, Félix A Rey, Yves Gaudin
1CNRS, Unité Mixte de Recherche (UMR) 2472, Institut National de la Recherche Agronomique (INRA), UMR 1157, Institut Fédératif de Recherche 115, Laboratoire de Virologie Moléculaire et Structurale, 91198, Gif sur Yvette, France.
Science (New York, N.Y.)
|February 10, 2007
Resumen
La glicoproteína G (G) del virus de la estomatitis vesicular sufre un cambio estructural inducido por un pH bajo para fusionar las membranas. Su estructura de prefusión revela una forma de trípode, exponiendo bucles de fusión para la interacción de la membrana viral y desencadenando la fusión de la membrana.
Área de la Ciencia:
- Virología Virología.
- Biología Estructural Biología estructural.
- La bioquímica es la bioquímica.
Sus antecedentes:
- La glicoproteína G (G) del virus de la estomatitis vesicular (VSV) es esencial para la entrada viral.
- La fusión de la membrana es un paso crítico en la infección viral, mediada por las glicoproteínas virales.
- La comprensión de la dinámica estructural de G es clave para elucidar los mecanismos de entrada de VSV.
Objetivo del estudio:
- Para determinar la estructura de alta resolución de la forma de prefusión de VSV Glicoproteína G.
- Para dilucidar las reorganizaciones estructurales involucradas en la transición fusogénica inducida por un bajo pH.
- Proponer una vía para los cambios conformacionales que conducen a la fusión de membranas.
Principales métodos:
- Se utilizó cristalografía de rayos X para determinar la estructura de la glicoproteína G. de la prefusión.
- Se realizó una comparación estructural entre las formas de prefusión y postfusión de la glicoproteína G.
- Análisis de las características estructurales relacionadas con los bucles de fusión y los sitios antigénicos.
Principales resultados:
- La estructura de prefusión de la glicoproteína G fue determinada a una resolución de 3.0 angstroms.
- La glicoproteína G adopta una conformación similar a un trípode en su estado de prefusión, con bucles de fusión expuestos.
- La transición fusogénica implica una extensa reorganización estructural, lo que sugiere un mecanismo cooperativo para la fusión de membranas.
Conclusiones:
- La estructura de la glicoproteína G de prefusión proporciona información sobre el estado inicial de la maquinaria de fusión.
- La vía estructural identificada pone de relieve los cambios conformacionales significativos requeridos para la fusión de membranas.
- Es probable que la acción cooperativa de múltiples moléculas de glicoproteína G esté involucrada en la inducción de la fusión de la membrana viral.
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