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Spatial and Temporal Analysis of Active ERK in the C. elegans Germline
Published on: November 29, 2016
Regulación de Cdc25C por las quinasas ERK-MAP durante la transición G2/M
Ruoning Wang1, Guangan He, Mayra Nelman-Gonzalez
1Department of Experimental Therapeutics, The University of Texas M. D. Anderson Cancer Center, Houston, TX 77030, USA.
Cell
|March 27, 2007
Resumen
Las vías de la proteína quinasa activada por mitógeno (MAPK) son cruciales para la progresión del ciclo celular. Este estudio revela que las quinasas ERK-MAP directamente fosforilan y activan la Cdc25 fosfatasa, un regulador clave de la transición G(2) / M en los ciclos celulares tanto meioticos como mitóticos.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología Molecular Biología Molecular
- La bioquímica es la bioquímica.
Sus antecedentes:
- La transición de fase G(2) / M está regulada por la proteína fosfatasa Cdc25, que requiere activación a través de la fosforilación.
- Si bien la Cdc2/ciclina B y la polo-like kinase (PLK) pueden activar la Cdc25 in vitro, su actividad por sí sola es insuficiente para explicar la activación de la Cdc25 durante la inducción de la fase M.
Objetivo del estudio:
- Para investigar el papel de la p42 MAP quinasa (MAPK), el ortólogo Xenopus de ERK2, en la activación de Cdc25 durante la transición G(2) / M.
- Para determinar si las quinasas ERK-MAP están involucradas en la activación de Cdc25 en células de mamíferos.
Principales métodos:
- Análisis de las interacciones proteicas y la fosforilación en extractos de huevo y ovocitos de Xenopus.
- Inhibición de la activación de ERK en una línea celular de mamíferos para evaluar su efecto en la fosforilación y activación de Cdc25C.
Principales resultados:
- p42 MAPK fue identificado como una importante quinasa fosforiladora de Cdc25 en extractos de huevo de Xenopus detenidos en fase M.
- En los ovocitos de Xenopus, p42 MAPK interactúa con Cdc25 y lo fosforila en sitios específicos (T48, T138, S205) durante la inducción meiótica, mejorando su actividad de fosfatasa.
- En las células de mamíferos, ERK1/2 interactúa con Cdc25C y lo fosforila en T48 durante la mitosis. La inhibición de la activación de ERK deterioró parcialmente la activación de Cdc25C y la inducción mitótica.
Conclusiones:
- Las quinasas ERK-MAP están directamente involucradas en la activación de Cdc25 durante la transición G(2)/M.
- Este mecanismo se conserva en todas las especies, destacando el papel fundamental de las quinasas ERK-MAP en la regulación del ciclo celular.
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