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Dinámica del estado excitado ultrarrápido de la proteína fotoswitchable Dronpapa

Eduard Fron1, Cristina Flors, Gerd Schweitzer

  • 1Department of Chemistry and Institute for Nanoscale Physics and Chemistry (INPAC), Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium.

Journal of the American Chemical Society
|March 28, 2007
PubMed
Resumen

No abstract available in PubMed .

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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