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La fosforilación de Erp1 por p90rsk es necesaria para la detención del factor citostático en los huevos de Xenopus
Tomoko Nishiyama1, Keita Ohsumi, Takeo Kishimoto
1Laboratory of Cell and Developmental Biology, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan.
Nature
|April 6, 2007
Resumen
Mos y Erp1/Emi2 colaboran para mantener la detención del factor citostático (FCS) en los huevos de Xenopus. La fosforilación dependiente de mos estabiliza Erp1/Emi2, mejorando su inhibición del complejo promotor de anafase/ciclosoma (APC/C).
Área de la Ciencia:
- Biología celular Biología celular.
- Biología del desarrollo Biología del desarrollo.
- Biología Molecular Biología Molecular
Sus antecedentes:
- La detención de los huevos de los vertebrados en la metafase de la meiosis II debido al factor citostático (CSF).
- La actividad del LCR inhibe el complejo/ciclosoma promotor de la anafase (APC/C), previniendo la degradación de la ciclina.
- Erp1/Emi2 y Mos son proteínas clave involucradas en el establecimiento y mantenimiento del arresto de la FCS.
Objetivo del estudio:
- Para aclarar la relación entre Mos y Erp1/Emi2 en CSF arrest.
- Para investigar el papel de la fosforilación dependiente de Mos de Erp1/Emi2.2.
Principales métodos:
- Análisis de Erp1/Emi2 como sustrato para p90rsk en extractos de huevo de Xenopus.
- Análisis semi-cuantitativo de la actividad Erp1/Emi2 en extractos de huevo detenidos por el LCR.
- Investigando la unión Erp1/Emi2 a la caja de destrucción APC/C.
Principales resultados:
- La fosforilación dependiente del mos de Erp1/Emi2 por p90rsk en sitios específicos es crucial para estabilizar Erp1/Emi2 y establecer la detención de CSF.
- La fosforilación mejora la actividad de Erp1/Emi2 en el mantenimiento de la detención de la metafase, pero no la genera.
- La fosforilación dependiente del musgo mejora la unión de Erp1/Emi2 a la APC/C, inhibiendo la degradación de la ciclina B.
Conclusiones:
- Mos y Erp1/Emi2 trabajan juntos para establecer y mantener la detención de la metafase II en los huevos de Xenopus.
- Este estudio proporciona un mecanismo molecular que une Mos y Erp1/Emi2 para la detención del LCR en huevos de vertebrados no fertilizados.
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