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El canal neuronal NALCN contribuye a la permeabilidad del sodio en reposo y es necesario para el ritmo respiratorio
Boxun Lu1, Yanhua Su, Sudipto Das
1Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA.
Cell
|April 24, 2007
Resumen
La proteína NALCN forma la conductividad crucial de las fugas de sodio (Na+), esencial para la excitabilidad del sistema nervioso. Este descubrimiento identifica el NALCNCN.
Área de la Ciencia:
- La neurociencia es la neurociencia.
- Biología Molecular Biología Molecular
- Fisiología del canal iónico Fisiología del canal iónico
Sus antecedentes:
- La excitabilidad basal del sistema nervioso está determinada por las permeabilidades de Na+ en reposo.
- La identidad molecular de la conductancia de fugas de Na + resistente a la tetrodotoxina (TTX) y al cesio (Cs +) sigue siendo desconocida.
Objetivo del estudio:
- Para identificar los componentes moleculares responsables de la conductancia de fugas de Na+ resistente a TTX y Cs+.
- Para aclarar el papel fisiológico de esta conductancia en la función neuronal y la supervivencia.
Principales métodos:
- Se utilizaron ratones mutantes NALCN para investigar la función de la proteína NALCN.
- Se realizaron grabaciones electrofisiológicas (tronco cerebral-medula espinal, neuronas del hipocampo) para evaluar el disparo neuronal y los potenciales de membrana.
- Se analizó el efecto de la concentración extracelular de Na+ en los potenciales de membrana en reposo en las neuronas mutantes.
Principales resultados:
- Identificó el NALCN, un nuevo canal catiónico no selectivo e independiente del voltaje, como la fuente de la conductancia de la fuga de Na+.
- Los ratones mutantes NALCN exhiben un ritmo respiratorio interrumpido y letalidad perinatal.
- Ausencia de corriente de fuga de Na+ resistente a TTX y Cs+ y potencial de membrana de reposo alterado en las neuronas mutantes NALCN.
Conclusiones:
- NALCN forma el fondo de Na + conductividad de fuga, crítico para el control de la excitabilidad neuronal.
- Este hallazgo revela el papel esencial del NALCN en el control respiratorio y la función neuronal.
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