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La isomerización cis-trans del prolyl inducida por la fuerza en polipéptidos similares a la elastina.

Alexei Valiaev¹, Dong Woo Lim, Terrence G Oas

¹Department of Mechanical Engineering and Materials Science, and Center for Biologically Inspired Materials and Materials Systems, Duke University, Durham, NC 27708, USA.

Journal of the American Chemical Society

|May 2, 2007

Resumen

La espectroscopia de fuerza de una sola molécula reveló que los polipéptidos similares a la elastina (ELPs) experimentan un alargamiento independiente de la temperatura debido a la isomerización cis-trans de prolyl inducida por la fuerza. Este hallazgo ofrece un nuevo método para estudiar este cambio conformacional clave en los polipéptidos.

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Área de la Ciencia:

La biofísica es la biofísica.
La ciencia de los polímeros es la ciencia de los polímeros.
Biología Estructural Biología estructural.

Sus antecedentes:

Los polipéptidos similares a la elastina (ELP) son polímeros sensibles al estímulo con secuencias VPGXG repetitivas.
La mecánica de conformación de los ELP suele estudiarse bajo diversas condiciones de disolvente.

Objetivo del estudio:

Investigar las transiciones extensionales inexplicables observadas en las curvas de fuerza-extensión del ELP.
Identificar el mecanismo molecular detrás de los cambios conformacionales inducidos por la fuerza en los ELPs.

Principales métodos:

Espectroscopia de fuerza de una sola molécula (SMFS) para medir curvas de fuerza-extensión.
Experimentos de control con el uso de poli (L-prolina) y poli (L-lisina).
Simulaciones de Monte Carlo utilizando un modelo de dos estados y acoplado elásticamente.

Principales resultados:

Se observaron transiciones extensionales independientes de la temperatura en ELPs que no se explican con modelos estándar de polímeros.
Identificó la isomerización cis-trans peptidyl-prolyl inducida por la fuerza como la causa de la elongación.
Se han demostrado transiciones similares en poli (L-prolina) pero no en poli (L-lisina).
Los experimentos de histeresis sugirieron el agotamiento del estado de prolina cis.

Conclusiones:

La isomerización cis-trans del prolyl inducida por la fuerza es un mecanismo clave en los cambios de conformación del ELP.
SMFS se puede utilizar para analizar la isomerización prolyl cis-trans en polipéptidos.
Este estudio proporciona la primera demostración de la isomerización cis-trans de prolyl inducida por la fuerza en polipéptidos que contienen prolina.