Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
Translational Regulation01:29

Translational Regulation

Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
Bacterial RNA Polymerase00:43

Bacterial RNA Polymerase

Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
Bacterial RNA Polymerase00:43

Bacterial RNA Polymerase

Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
Nucleic Acid Structure01:25

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

The next-generation CAR-T therapy landscape.

Nature reviews. Drug discovery·2023
Same author

Manufacturing innovation to drive down cell therapy costs.

Trends in biotechnology·2023
Same author

Characterization of Engineered PreQ1 Riboswitches for Inducible Gene Regulation in Mycobacteria.

Journal of bacteriology·2017
Same author

RNA synthetic biology: from the test tube to cells and back again.

ACS synthetic biology·2015
Same author

A family of synthetic riboswitches adopts a kinetic trapping mechanism.

Nucleic acids research·2014
Same author

Riboswitches for intracellular study of genes involved in Francisella pathogenesis.

mBio·2012

Video Experimental Relacionado

Updated: Jul 15, 2026

A Non-Coding Small RNA MicC Contributes to Virulence in Outer Membrane Proteins in Salmonella Enteritidis
06:30

A Non-Coding Small RNA MicC Contributes to Virulence in Outer Membrane Proteins in Salmonella Enteritidis

Published on: January 27, 2021

Bacteria guía con pequeñas moléculas y ARN.

Shana Topp1, Justin P Gallivan

  • 1Department of Chemistry and Center for Fundamental and Applied Molecular Evolution, Emory University, 1515 Dickey Drive, Atlanta, Georgia 30322, USA.

Journal of the American Chemical Society
|May 8, 2007
PubMed
Resumen

Los científicos reprogramaron las bacterias E. coli para seguir nuevas señales químicas utilizando un riboswitch sintético. Este avance permite una localización bacteriana precisa para aplicaciones en biorremediación y biología sintética.

Más Videos Relacionados

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria
08:34

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria

Published on: February 23, 2021

Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution
10:27

Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution

Published on: July 8, 2019

Videos de Experimentos Relacionados

Last Updated: Jul 15, 2026

A Non-Coding Small RNA MicC Contributes to Virulence in Outer Membrane Proteins in Salmonella Enteritidis
06:30

A Non-Coding Small RNA MicC Contributes to Virulence in Outer Membrane Proteins in Salmonella Enteritidis

Published on: January 27, 2021

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria
08:34

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria

Published on: February 23, 2021

Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution
10:27

Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution

Published on: July 8, 2019

Área de la Ciencia:

  • Microbiología Microbiología.
  • Biología sintética Biología sintética.
  • Biotecnología La biotecnología es la biotecnología.

Sus antecedentes:

  • Las bacterias navegan por entornos químicos utilizando vías de señalización complejas y motores moleculares.
  • La reprogramación de las bacterias para detectar nuevas moléculas es un desafío debido a la intrincada señalización y las limitaciones de la ingeniería de proteínas.

Objetivo del estudio:

  • Desarrollar un método para reprogramar bacterias para que sigan señales químicas completamente nuevas.
  • Demostrar el uso de riboswitches sintéticos para el reconocimiento de nuevos ligandos en bacterias.

Principales métodos:

  • Diseñado E. coli con un riboswitch sintético para detectar un nuevo ligando específico.
  • Utilizó el sistema de riboswitch para guiar la quimiotaxis bacteriana hacia la señal química objetivo.

Principales resultados:

  • Se ha reprogramado con éxito E. coli para que exhiba quimiotaxis hacia una señal química completamente nueva.
  • Logró la localización precisa de las bacterias a la nueva señal química utilizando el sistema de riboswitch sintético.

Conclusiones:

  • Los riboswitches sintéticos ofrecen una alternativa viable a la ingeniería de proteínas para reprogramar la quimiotaxis bacteriana.
  • Este enfoque abre nuevas vías para las bacterias en aplicaciones de biorremediación, bionanotecnología y biología sintética.