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Presynaptically Silent Synapses Studied with Light Microscopy
11:02

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Published on: January 4, 2010

Degradado en el silencio.

Elizabeth H Bayne1, Sharon A White, Robin C Allshire

  • 1Wellcome Trust Centre for Cell Biology, University of Edinburgh, Kings Buildings, Michael Swann Building, Mayfield Road, Edinburgh, EH9 3JR, UK.

Cell
|May 22, 2007
PubMed
Resumen

La maquinaria de interferencia de ARN se dirige directamente a las transcripciones transgénicas en la levadura de fisión, vinculando el silenciamiento a un complejo similar a TRAMP. Esto sugiere que la degradación de la transcripción independiente del ARNi contribuye al silenciamiento del gen de la heterocromatina.

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Área de la Ciencia:

  • Biología Molecular Biología Molecular
  • Genética La genética.
  • La epigenética es la epigenética.

Sus antecedentes:

  • En la levadura de fisión, la formación de heterocromatina mediada por la interferencia del ARN (RNAi) silencia los transgenes en los centrómeros.
  • Se están investigando los mecanismos precisos que vinculan el ARNi con la heterocromatina y el silenciamiento transgénico.

Objetivo del estudio:

  • Para investigar los objetivos directos de la maquinaria de interferencia de ARN en el silenciamiento transgénico.
  • Explorar el papel de los complejos de proteínas en la formación de heterocromatina dependiente de ARNi y el silenciamiento de genes.

Principales métodos:

  • Análisis de las vías de interferencia del ARN en la levadura de fisión.
  • Investigación de la orientación del transcrito transgénico por la maquinaria de RNAi.
  • Caracterización de los complejos proteicos implicados en la degradación de los transcritos.

Principales resultados:

  • La maquinaria de RNAi se dirige directamente a las transcripciones transgénicas para su degradación.
  • Un complejo proteico similar al complejo TRAMP en la levadura en ciernes está relacionado con el silenciamiento transgénico.
  • Este complejo promueve la degradación de la transcripción a través del exosoma.

Conclusiones:

  • La maquinaria de interferencia de ARN interactúa directamente con las transcripciones transgénicas.
  • El silenciamiento transgénico implica una degradación compleja similar a TRAMP y mediada por exosomas.
  • Las vías de degradación de transcripción independientes del ARN-i pueden contribuir al silenciamiento de genes mediado por la heterocromatina.