Search research articles

ACERCA DE JoVE

Visión General Liderazgo Blog Centro de Ayuda JoVE

AUTORES

Proceso de Publicación Consejo Editorial Alcance y Políticas Revisión por Pares Preguntas Frecuentes Enviar

BIBLIOTECARIOS

Testimonios Suscripciones Acceso Recursos Consejo Asesor de Bibliotecas Preguntas Frecuentes

INVESTIGACIÓN

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archivo

EDUCACIÓN

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Centro de Recursos para Profesores Sitio de Profesores

Términos y Condiciones de Uso

Política de Privacidad

Videos de Conceptos Relacionados

In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

In vitro Mutagenesis

In vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

Equivalence: In Vitro and In Vivo Bioequivalence

Equivalence: In Vitro and In Vivo Bioequivalence

Bioequivalence studies are crucial in evaluating whether new drugs can match an approved one regarding pharmacological effects and clinical performance. These studies test if drugs, despite different dosage forms, share identical plasma concentration-time profiles. Three types of equivalence are central to these studies: chemical, pharmaceutical, and therapeutic. Chemical equivalence indicates that two or more drug products contain identical active ingredients in equal amounts. Pharmaceutical...

In Vitro Drug Dissolution: Compendial Testing Models I

In Vitro Drug Dissolution: Compendial Testing Models I

Compendial dissolution methods are standardized procedures defined by pharmacopeias to evaluate the rate at which a drug dissolves in a specific medium. These methods ensure batch-to-batch consistency, enable quality control, and support the prediction of drug bioavailability. They are critical for both immediate and modified-release drug products.The apparatuses used for dissolution testing differ in their design and mechanical function, but all aim to simulate the physiological environment of...

In Vitro Drug Dissolution: Compendial Testing Models II

In Vitro Drug Dissolution: Compendial Testing Models II

Various dissolution methods are utilized to assess a drug’s dissolution rate, including the flow-through cell, paddle-over-disk, cylinder, and reciprocating disk methods.The flow-through cell apparatus (USP (United States Pharmacopeia) method 4) comprises a reservoir for the dissolution medium and a pump that propels the medium through the cell containing the test sample. This method is crucial for assessing modified-release dosage forms with minimally soluble active ingredients, maintaining...

In Vitro Drug Dissolution: Alternative Methods

In Vitro Drug Dissolution: Alternative Methods

Alternative drug dissolution methods include the rotating bottle, intrinsic dissolution test, peristalsis, and the Franz diffusion cell method. The rotating bottle method involves meticulously rotating tightly capped controlled-release beads in a temperature-controlled bath. Periodic decanting of samples allows for residue assay, followed by refilling with fresh medium and testing at various pH levels to emulate the gastrointestinal tract conditions.In contrast, the intrinsic dissolution test...

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por

Same author

Bayesian-Steered Structure Prediction of Mechanical Biomolecules Using Twisted Diffusion.

bioRxiv : the preprint server for biology·2026

Same author

In-Situ ssDNA Isolation from dsDNA Sources as a Streamlined Pathway to DNA Origami Assembly and Testing.

bioRxiv : the preprint server for biology·2026

Same author

PySteMoDA: An Open-Source Python Package for the Analysis of Steered Molecular Dynamics Simulations Data.

bioRxiv : the preprint server for biology·2026

Same author

Structures of invertebrate PEZO-1 isoforms with a compact architecture and a dispensable pore-distal N-terminal blade.

Cell reports·2026

Same author

Groove architecture controls lipid scrambling in simulations of protein and model systems.

bioRxiv : the preprint server for biology·2025

Same author

TMC1 and TMC2 are cholesterol-dependent scramblases that regulate membrane homeostasis in auditory hair cells.

bioRxiv : the preprint server for biology·2025

Search research articles

Video Experimental Relacionado

Updated: Jul 14, 2026

A Combined 3D Tissue Engineered In Vitro/In Silico Lung Tumor Model for Predicting Drug Effectiveness in Specific Mutational Backgrounds

A Combined 3D Tissue Engineered In Vitro/In Silico Lung Tumor Model for Predicting Drug Effectiveness in Specific Mutational Backgrounds

Published on: April 6, 2016

Experimentos con una sola molécula in vitro e in silico.

Marcos Sotomayor¹, Klaus Schulten

¹Department of Physics, University of Illinois at Urbana-Champaign, and Beckman Institute for Advanced Science and Technology, 405 North Mathews Avenue, Urbana, IL 61801, USA.

Science (New York, N.Y.)

|May 26, 2007

Resumen

Las simulaciones de dinámica molecular proporcionan información a nivel atómico sobre la mecánica de las proteínas, complementando los experimentos in vitro. Estos estudios "in silico" revelan los mecanismos moleculares detrás de la elasticidad de proteínas clave como la titina y la fibronectina.

Más Videos Relacionados

In Vitro Transcription Assays and Their Application in Drug Discovery

In Vitro Transcription Assays and Their Application in Drug Discovery

Published on: September 20, 2016

The Lambda Select cII Mutation Detection System

The Lambda Select cII Mutation Detection System

Published on: April 26, 2018

Videos de Experimentos Relacionados

Last Updated: Jul 14, 2026

A Combined 3D Tissue Engineered In Vitro/In Silico Lung Tumor Model for Predicting Drug Effectiveness in Specific Mutational Backgrounds

A Combined 3D Tissue Engineered In Vitro/In Silico Lung Tumor Model for Predicting Drug Effectiveness in Specific Mutational Backgrounds

Published on: April 6, 2016

In Vitro Transcription Assays and Their Application in Drug Discovery

In Vitro Transcription Assays and Their Application in Drug Discovery

Published on: September 20, 2016

The Lambda Select cII Mutation Detection System

The Lambda Select cII Mutation Detection System

Published on: April 26, 2018

Área de la Ciencia:

La biofísica es la biofísica.
Biología computacional Biología computacional.
Biología Estructural Biología estructural.

Sus antecedentes:

Los experimentos de fuerza de una sola molécula in vitro caracterizan la respuesta mecánica a escala nanométrica de la materia biológica.
Estos experimentos no aclaran completamente los mecanismos moleculares de la función mecánica.
Las simulaciones de dinámica molecular (DM) ofrecen un enfoque complementario para estudiar estos mecanismos.

Objetivo del estudio:

Para revisar las investigaciones recientes que utilizan simulaciones dirigidas de MD.
Para ilustrar cómo los experimentos in silico proporcionan información sobre la mecánica de las proteínas.
Para resaltar la aplicación de simulaciones de MD en la comprensión de la elasticidad de las proteínas.

Principales métodos:

Simulaciones de dinámica molecular dirigida (DMD) de modelos estructurales atómicos.
Experimentos de una sola molécula in silico para sondear la respuesta macromolecular.
Análisis de investigaciones recientes en la mecánica de las proteínas.

Principales resultados:

Las simulaciones de SMD revelan mecanismos moleculares que subyacen a la elasticidad de las proteínas.
Conocimiento de la respuesta mecánica de las repeticiones de titina, fibronectina, espectrina y ankyrina.
Demostración del SMD como una poderosa herramienta que complementa los experimentos in vitro.

Conclusiones:

Los experimentos in silico de una sola molécula son cruciales para comprender la función mecánica de las proteínas.
Las simulaciones dirigidas de MD proporcionan conocimientos a nivel atómico que guían la investigación experimental.
Este enfoque mejora nuestra comprensión de los diversos sistemas de proteínas, incluidos los componentes de la matriz citosquelética y extracelular.