Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

RNA Structure01:23

RNA Structure

69.1K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
69.1K
RNA Stability01:53

RNA Stability

31.7K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
31.7K
Transfer RNA Synthesis02:36

Transfer RNA Synthesis

10.3K
One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
10.3K
Riboswitches01:56

Riboswitches

8.0K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.0K
RNA Structure01:19

RNA Structure

6.6K
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
6.6K
Nucleic Acid Structure01:25

Nucleic Acid Structure

8.1K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
8.1K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Copy-back RNA synthesis by coronavirus polymerase requires helicase activity and is stimulated by remdesivir and molnupiravir.

Science advances·2026
Same author

Selective targeting of a histone-like silencer Sfx to the R6K conjugal transfer operon.

Nucleic acids research·2026
Same author

The ω subunit stabilizes transcribing RNA polymerase to balance processivity and collision resolution.

bioRxiv : the preprint server for biology·2026
Same author

Exploration of the structural and functional diversity in the metamorphic RfaH subfamily.

bioRxiv : the preprint server for biology·2026
Same author

Selective targeting of a histone-like silencer Sfx to the R6K conjugal transfer operon.

bioRxiv : the preprint server for biology·2026
Same author

Template switching by coronavirus polymerase requires helicase activity and is stimulated by remdesivir and molnupiravir.

bioRxiv : the preprint server for biology·2025

Video Experimental Relacionado

Updated: May 6, 2026

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
10:52

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

Published on: September 28, 2017

7.4K

Bases estructurales para la carga del sustrato en la ARN polimerasa bacteriana.

Dmitry G Vassylyev1, Marina N Vassylyeva, Jinwei Zhang

  • 1Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Schools of Medicine and Dentistry, 402B Kaul Genetics Building, 720 20th Street South, Birmingham, Alabama 35294, USA. dmitry@uab.edu

Nature
|June 22, 2007
PubMed
Resumen

El mecanismo de replegamiento del bucle gatillo en la ARN polimerasa es vital para la carga del sustrato. Los estudios estructurales revelan distintos estados de preinserción e inserción, ofreciendo objetivos para el desarrollo de antibióticos.

Más Videos Relacionados

An Assay for Quantifying Protein-RNA Binding in Bacteria
07:02

An Assay for Quantifying Protein-RNA Binding in Bacteria

Published on: June 12, 2019

5.9K
Antibody-Free Assay for RNA Methyltransferase Activity Analysis
08:31

Antibody-Free Assay for RNA Methyltransferase Activity Analysis

Published on: July 9, 2019

6.8K

Videos de Experimentos Relacionados

Last Updated: May 6, 2026

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
10:52

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

Published on: September 28, 2017

7.4K
An Assay for Quantifying Protein-RNA Binding in Bacteria
07:02

An Assay for Quantifying Protein-RNA Binding in Bacteria

Published on: June 12, 2019

5.9K
Antibody-Free Assay for RNA Methyltransferase Activity Analysis
08:31

Antibody-Free Assay for RNA Methyltransferase Activity Analysis

Published on: July 9, 2019

6.8K

Área de la Ciencia:

  • La bioquímica es la bioquímica.
  • Biología Estructural Biología estructural.
  • Biología Molecular Biología Molecular

Sus antecedentes:

  • La comprensión de la carga del sustrato de la ARN polimerasa es clave para la transcripción.
  • El mecanismo exacto sigue siendo objeto de debate.

Objetivo del estudio:

  • Para dilucidar el mecanismo de carga de sustrato en la polimerasa de ARN multisubunitaria.
  • Para determinar la base estructural de la unión y la inhibición del sustrato.

Principales métodos:

  • Cristalografía de rayos X con una resolución de 3.0-A.
  • Pruebas bioquímicas. ensayos bioquímicos. ensayos bioquímicos. ensayos bioquímicos. ensayos bioquímicos. ensayos bioquímicos. ensayos bioquímicos. ensayos bioquímicos. ensayos bioquímicos. ensayos bioquímicos. ensayos bioquímicos.

Principales resultados:

  • Se determinaron las estructuras del complejo de alargamiento de Thermus thermophilus (CE) con AMPcPP y AMPcPP/estreptolidigina.
  • AMPcPP se une en un sitio de "inserción" activo a través del replegamiento del bucle de activación (TL).
  • La estreptolidigina estabiliza un estado de "preinserción" inactivo desplazando el TL.

Conclusiones:

  • El replegamiento de TL es esencial para la catálisis de la ARN polimerasa.
  • Un mecanismo de preinserción / inserción de dos pasos para la carga del sustrato puede ser universal.
  • El estado de preinserción es un objetivo potencial para nuevos antibióticos.