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Estructura y organización de las proteínas de capa en la jaula COPII
Stephan Fath1, Joseph D Mancias, Xiping Bi
1Howard Hughes Medical Institute and the Structural Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.
Cell
|July 3, 2007
Resumen
El abrigo de la COPII.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología Estructural Biología estructural.
- La bioquímica es la bioquímica.
Sus antecedentes:
- Las vesículas recubiertas de COPII median el transporte de proteínas desde el retículo endoplasmático.
- La capa COPII comprende componentes Sec23/24-Sar1 de selección de carga y Sec13/31 de deformación de membrana.
Objetivo del estudio:
- Para aclarar la arquitectura molecular de la jaula COPII.
- Para entender el mecanismo de polimerización de la unidad de ensamblaje Sec13/31.
Principales métodos:
- Análisis cristalográfico de la unidad de ensamblaje Sec13/31.
- Modelado molecular que integra estructuras cristalinas con datos de microscopía electrónica.
Principales resultados:
- La unidad de ensamblaje Sec13/31 es una varilla con dominios de hélice beta en cada extremo.
- Un modelo molecular revela los vértices de la jaula COPII formados por cuatro hélices beta Sec31.
- La estructura de la jaula propuesta carece de interdigitación, a diferencia de las jaulas de clatrina.
Conclusiones:
- La estructura de la jaula COPII está determinada por la convergencia específica de las unidades de ensamblaje Sec13/31.
- Una bisagra central en la unidad de ensamblaje permite la adaptación a diferentes curvaturas de la membrana.
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