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Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues
Published on: February 17, 2017
El enlace alfa,alfa-(1-->1) de la trehalosa es clave para la preservación anidrobiótica
Fernando Albertorio1, Vanessa A Chapa, Xin Chen
1Department of Chemistry, 3255 TAMU, Texas A&M University, College Station, Texas 77843, USA.
Journal of the American Chemical Society
|August 7, 2007
Resumen
La alfa, alfa-trehalosa y la alfa, alfa-galacto-trehalosa conservan eficazmente las bicapas lipídicas con soporte sólido (SLB) durante el secado. Su enlace específico es clave, a diferencia de otros azúcares que causan la destrucción de las dos capas.
Área de la Ciencia:
- La biofísica es la biofísica.
- Ciencia de los materiales Ciencia de los materiales.
Sus antecedentes:
- Las bicapas lipídicas con soporte sólido (SLB) son modelos cruciales para las membranas celulares, pero son sensibles a la deshidratación.
- La preservación de la integridad del SLB al retirar el agua es esencial para su almacenamiento y aplicación.
Objetivo del estudio:
- Evaluar la eficacia de varios sacarídeos en la protección de las bicapas lipídicas con soporte sólido (SLB) de la exposición al aire.
- Determinar las propiedades estructurales y químicas de los sacarídeos que confieren capacidades protectoras.
Principales métodos:
- Análisis comparativo de seis disacáridos y glucosa para la preservación de SLB.
- Evaluación de la retención de la estructura de dos capas después del secado en presencia de diferentes azúcares.
- Medición de la temperatura de transición de fase de cristalino líquido a gel de las bicapas de fosfolípidos con varios azúcares.
Principales resultados:
- Los disacáridos con un enlace alfa,alfa-(1-->1) (por ejemplo, alfa,alfa-trehalosa) conservan efectivamente la estructura SLB.
- Los sacáridos que se cristalizan en estructuras abiertas (sacarosa, maltosa, lactosa, glucosa) y alfa, beta-trehalosa no pudieron proteger a las SLB.
- Los azúcares vinculados covalentemente en los lípidos (gangliosida GM1, lactosilo fosfatidil etanolamina, glucosilcerebrosida) también fueron ineficaces.
- Solo la alfa, alfa-trehalosa y la alfa, alfa-galacto-trehalosa disminuyeron la temperatura de transición de fase; otros la aumentaron.
Conclusiones:
- El enlace axial-axial en alfa, alfa-disacáridos es crítico para mantener la integridad de la SLB durante la deshidratación.
- La conformación y el tipo de enlace de los sacáridos influyen significativamente en su capacidad para estabilizar las bicapas lipídicas.
- La comprensión de estas interacciones puede guiar el desarrollo de mejores métodos para la preservación de la bicapa lipídica.
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