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Divergencia de los sitios de unión del factor de transcripción entre especies de levaduras relacionadas
Anthony R Borneman1, Tara A Gianoulis, Zhengdong D Zhang
1Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06511, USA.
Science (New York, N.Y.)
|August 11, 2007
Resumen
Las diferencias de regulación genética entre las especies de levadura revelan una rápida evolución de los sitios de unión del factor de transcripción. Esta divergencia excede significativamente los cambios en los propios genes, impulsando la evolución de las especies.
Área de la Ciencia:
- Biología evolutiva Biología evolutiva.
- Biología molecular La biología molecular.
- Genética La genética.
Sus antecedentes:
- Comprender la regulación génica entre especies es clave para explicar la diversidad fenotípica y la evolución.
- Los factores de transcripción (TF) juegan un papel crítico en la regulación de los genes.
Objetivo del estudio:
- Para investigar la divergencia de los sitios de unión del factor de transcripción entre las especies de levadura.
- Determinar el papel de la evolución del sitio de unión de TF en la divergencia de especies.
Principales métodos:
- Se utilizó inmunoprecipitación de cromatina (ChIP) seguida de análisis de microarrays de ADN.
- Los sitios de unión para los reguladores seudohifales Ste12 y Tec1 fueron analizados en Saccharomyces cerevisiae, S. mikatae y S. bayanus.
Principales resultados:
- La mayoría de los sitios de unión de Ste12 y Tec1 han divergido entre las especies de levadura estudiadas.
- La divergencia del sitio de unión excedió significativamente la variación entre especies en los genes ortólogos.
- Un subconjunto de los objetivos de la Etapa 12 estaban vinculados de forma única en S. mikatae y S. bayanus, lo que sugiere una especialización de la vía.
Conclusiones:
- Los sitios de unión al factor de transcripción evolucionan mucho más rápido que el contenido genético ortólogo.
- La divergencia en la regulación génica a través de la unión a TF es un importante impulsor de la divergencia evolutiva entre especies.
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