Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Non-gated Ion Channels01:24

Non-gated Ion Channels

Ion channels are specialized proteins on the plasma membrane that allow charged ions to pass down their electrochemical gradient. Their main function is to maintain the membrane potential which is critical for cell viability. These channels are either gated or non-gated and can transport more than a thousand ions within milliseconds for the cellular event to occur.
Compared to the gated ion channels, the non-gated channels, also known as leakage or passive channels, have no gating mechanism.
The Role of Ion Channels in Neuronal Computation01:19

The Role of Ion Channels in Neuronal Computation

A postsynaptic neuron usually receives numerous impulses from several other presynaptic neurons. The axon hillock of the postsynaptic neuron integrates all these signals and determines the likelihood of firing an action potential.
Sometimes a single EPSP is strong enough to induce an action potential in the postsynaptic neuron. However, multiple presynaptic inputs must often create EPSPs around the same time for the postsynaptic neuron to be sufficiently depolarized to fire an action potential.
Non-gated Ion Channels01:24

Non-gated Ion Channels

Ion channels are specialized proteins on the plasma membrane that allow charged ions to pass down their electrochemical gradient. Their main function is to maintain the membrane potential which is critical for cell viability. These channels are either gated or non-gated and can transport more than a thousand ions within milliseconds for the cellular event to occur.
Compared to the gated ion channels, the non-gated channels, also known as leakage or passive channels, have no gating mechanism.
Integration of Synaptic Events01:28

Integration of Synaptic Events

Synaptic integration mainly includes the summation of graded potentials. Graded potentials, regardless of their type, cause subtle alterations in membrane voltage, resulting in either depolarization or hyperpolarization. These incremental changes, when combined or summed, can propel the neuron toward its threshold. Consider, for example, a membrane experiencing a +15 mV shift, causing it to depolarize from -70 mV to -55 mV. In this scenario, graded potentials govern the membrane's ability to...
Voltage-gated Ion Channels01:26

Voltage-gated Ion Channels

Voltage-gated ion channels are transmembrane proteins that open and close in response to changes in the membrane potential. They are present on the membranes of all electrically excitable cells such as neurons, heart, and muscle cells.
Generally, all voltage-gated ion channels have a 'voltage-sensing domain' that spans the lipid bilayer. The charged residues in the sensor move in response to the membrane potential changes that open the channel allowing ions movement. There are several types of...
Regulation of Sodium and Potassium01:26

Regulation of Sodium and Potassium

The regulation of sodium and potassium ion concentrations in the human body is a complex process governed primarily by hormones such as aldosterone, antidiuretic hormone (ADH), and atrial natriuretic peptide (ANP).
Sodium Regulation
Sodium ions make up approximately 90% of extracellular cations, with a normal blood plasma concentration of 136–148 mEq/L. A decrease in blood volume and pressure triggers the release of renin from granular cells in the juxtaglomerular complex (JGC), primarily in...

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Inhibition of (interstitial) P2Y<sub>6</sub> receptors attenuates fibrosis progression.

Pflugers Archiv : European journal of physiology·2026
Same author

Inhibition of (interstitial) P2Y<sub>6</sub> receptors attenuates fibrosis progression.

Research square·2026
Same author

Opening closed inward rectifier potassium channel doors.

British journal of pharmacology·2026
Same author

Inhibition of (interstitial) P2Y<sub>6</sub> receptors attenuates renal fibrosis progression.

bioRxiv : the preprint server for biology·2026
Same author

Light-induced analgesia provides a drug-free optical method for pain relief via activation of TRAAK k<sup>+</sup> channels.

Nature communications·2026
Same author

SPARC: a structural pathogenicity algorithm for risk classification of hERG variants.

Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology·2025

Video Experimental Relacionado

Updated: Jun 22, 2026

Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80
12:28

Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80

Published on: May 3, 2015

¿La sumoilación controla los canales K+ de fondo de K2P1/TWIK1?

Sylvain Feliciangeli1, Saïd Bendahhou, Guillaume Sandoz

  • 1Institut de Pharmacologie Moléculaire et Cellulaire, CNRS UMR6097, Institut Paul Hamel, 660, route des lucioles, 06560 Valbonne, France.

Cell
|August 19, 2007
PubMed
Resumen

Un nuevo modelo sugiere que la excitabilidad celular está regulada por la sumoilación del canal K2P1. La mutación en un sitio específico (K274E) aumentó la corriente de K2P1, lo que indica un efecto de carga, no de sumoilación.

Más Videos Relacionados

In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity
09:45

In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity

Published on: January 29, 2018

Study of the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2 Using Western Blotting
10:08

Study of the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2 Using Western Blotting

Published on: December 9, 2022

Videos de Experimentos Relacionados

Last Updated: Jun 22, 2026

Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80
12:28

Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80

Published on: May 3, 2015

In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity
09:45

In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity

Published on: January 29, 2018

Study of the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2 Using Western Blotting
10:08

Study of the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2 Using Western Blotting

Published on: December 9, 2022

Área de la Ciencia:

  • La neurociencia es la neurociencia.
  • Biología Molecular Biología Molecular
  • Fisiología del canal iónico Fisiología del canal iónico

Sus antecedentes:

  • La excitabilidad celular es crucial para la función neuronal.
  • El canal de potasio de fondo K2P1 (TWIK1) está implicado en la regulación de la excitabilidad celular.
  • La sumoilación se ha propuesto como un mecanismo de regulación para los canales K2P1.

Objetivo del estudio:

  • Investigar el papel de la sumoylación en K274 en la regulación del canal K2P1.
  • Para determinar si la sumoilación K274 afecta la densidad de corriente del canal K2P1.
  • Explorar el mecanismo detrás de la regulación del canal K2P1 por K274.4.

Principales métodos:

  • Mutagénesis dirigida al sitio del canal K2P1 en la posición 274 (K274E y K274R).
  • Expresión heteróloga de los canales K2P1 de tipo silvestre y mutante en las células COS-7.
  • Grabaciones de pinzas de tensión de dos electrodos en ovocitos de Xenopus.
  • Análisis de la mancha occidental para detectar la sumoilación potencial.

Principales resultados:

  • La mutación K274E, pero no K274R, aumentó significativamente la densidad de corriente de K2P1.
  • El aumento observado en la densidad de corriente sugiere un efecto dependiente de la carga en la posición 274.
  • El análisis de Western blot no proporcionó evidencia de la sumoilación de K2P1 ni en las células COS-7 ni en los ovocitos.

Conclusiones:

  • El residuo K274 en los canales K2P1 influye en la densidad de corriente a través de un efecto de carga, no a través de la sumoyación.
  • El modelo propuesto de silenciamiento del canal K2P1 por sumoilación requiere una reevaluación.
  • Se necesita más investigación para dilucidar los mecanismos precisos que regulan la actividad del canal K2P1.