Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Multi-species Conserved Sequences02:51

Multi-species Conserved Sequences

Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale  studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved DNA...
Heterochromatin02:38

Heterochromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
Genomic DNA in Eukaryotes00:58

Genomic DNA in Eukaryotes

Eukaryotes have large genomes compared to prokaryotes. To fit their genomes into a cell, eukaryotic DNA is packaged extraordinarily tightly inside the nucleus. To achieve this, DNA is tightly wound around proteins called histones, which are packaged into nucleosomes that are joined by linker DNA and coil into chromatin fibers. Additional fibrous proteins further compact the chromatin, which is recognizable as chromosomes during certain phases of cell division.
Euchromatin01:01

Euchromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
Euchromatin01:01

Euchromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
Conserved Binding Sites01:49

Conserved Binding Sites

Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally analyses the...

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Protocol for genotyping cephalopod sex using a skin swab and quantitative PCR.

STAR protocols·2026
Same author

Servo-Actuated 3D-Printed Disposable Microvalves for Automated, Scalable Organoid Culture in Standard Incubators.

bioRxiv : the preprint server for biology·2026
Same author

Cloud-connected pluripotent stem cell platform enhances scientific identity in underrepresented students.

Stem cell reports·2026
Same author

MKado: a toolkit for McDonald-Kreitman tests of natural selection.

G3 (Bethesda, Md.)·2026
Same author

Ecotypes, <i>Wolbachia</i>, and urbanization shape <i>Culex pipiens</i> population structure in a West Nile virus hotspot.

bioRxiv : the preprint server for biology·2026
Same author

Diversity and divergence of two sympatric, sibling octopus species.

bioRxiv : the preprint server for biology·2026

Video Experimental Relacionado

Updated: Jul 13, 2026

Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease
09:20

Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease

Published on: February 1, 2022

En el genoma humano, los elementos ultraconservados son elementos ultraseleccionados.

Sol Katzman1, Andrew D Kern, Gill Bejerano

  • 1Department of Biomolecular Engineering, University of California, Santa Cruz, CA 95064, USA.

Science (New York, N.Y.)
|August 19, 2007
PubMed
Resumen

Los elementos ultraconservados en el genoma humano son secuencias de ADN altamente conservadas. Estas regiones experimentan una selección negativa más fuerte que los genes codificadores de proteínas, lo que desafía las hipótesis anteriores.

Más Videos Relacionados

Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells
14:26

Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells

Published on: April 4, 2016

Ultra-long Read Sequencing for Whole Genomic DNA Analysis
10:34

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

Videos de Experimentos Relacionados

Last Updated: Jul 13, 2026

Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease
09:20

Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease

Published on: February 1, 2022

Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells
14:26

Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells

Published on: April 4, 2016

Ultra-long Read Sequencing for Whole Genomic DNA Analysis
10:34

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

Área de la Ciencia:

  • La genómica es la genómica.
  • Biología evolutiva Biología evolutiva.
  • La bioinformática es la bioinformática.

Sus antecedentes:

  • Los elementos ultraconservados (UCE) son secuencias de ADN (>200 bp) idénticas en los genomas de humanos, ratones y ratas.
  • La mayoría de las UCE no codifican y se han conservado en mamíferos y aves durante más de 300 millones de años.
  • Los impulsores evolutivos de la conservación de la UCE son poco conocidos.

Objetivo del estudio:

  • Investigar las presiones selectivas que actúan sobre los elementos ultraconservados.
  • Para determinar si las UCE son puntos fríos mutacionales o bajo selección negativa.
  • Para comparar la intensidad de selección en UCEs con genes codificadores de proteínas.

Principales métodos:

  • Análisis del espectro de frecuencia del alelo derivado en UCE.
  • Genómica comparativa para identificar regiones conservadas.
  • Análisis bioinformático de datos de secuencia.

Principales resultados:

  • Los elementos ultraconservados no son puntos fríos mutacionales.
  • Las UCE están bajo una selección evolutiva negativa significativa.
  • La selección negativa en UCE es más fuerte que en los genes codificadores de proteínas.

Conclusiones:

  • La conservación extrema de las UCE es impulsada por una fuerte selección negativa.
  • Este hallazgo refuta la hipótesis del punto frío mutacional.
  • Las UCE representan regiones regulatorias o funcionales críticas bajo una intensa restricción evolutiva.