Search research articles

ACERCA DE JoVE

Visión General Liderazgo Blog Centro de Ayuda JoVE

AUTORES

Proceso de Publicación Consejo Editorial Alcance y Políticas Revisión por Pares Preguntas Frecuentes Enviar

BIBLIOTECARIOS

Testimonios Suscripciones Acceso Recursos Consejo Asesor de Bibliotecas Preguntas Frecuentes

INVESTIGACIÓN

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archivo

EDUCACIÓN

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Centro de Recursos para Profesores Sitio de Profesores

Términos y Condiciones de Uso

Política de Privacidad

Videos de Conceptos Relacionados

Centrosome Duplication

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...

Separation of Sister Chromatids

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...

The Spindle Assembly Checkpoint

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...

Anaphase Promoting Complex

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...

Catenins

Catenins

Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the adherens...

Centrosome Duplication

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por

Same author

Identification of a component of the tubulin poly-glutamylase complex required for phosphoinositide homeostasis and cilium assembly and maintenance.

Nature communications·2026

Same author

Automated segmentation of trunk musculature with a deep CNN trained from sparse annotations in radiation therapy patients with metastatic spine disease: an observational study.

Frontiers in bioengineering and biotechnology·2026

Same author

Metastatic Spine Disease Alters Vertebral Load-To-Strength Ratios in Cancer Patients Compared to Healthy Individuals.

JOR spine·2026

Same author

Targeting G1-S-checkpoint-compromised cancers with cyclin A/B RxL inhibitors.

Nature·2025

Same author

Spinal Instability Neoplastic Score as a Predictor of Vertebral Fracture in Patients Undergoing Radiation Therapy for Spinal Metastases: A Single-Institution Study.

Advances in radiation oncology·2025

Same author

PAX translocations remodel mitochondrial metabolism through altered leucine usage in rhabdomyosarcoma.

Cell·2025

Search research articles

Video Experimental Relacionado

Updated: May 13, 2026

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion

Published on: June 25, 2017

Cep97 y CP110 suprimen un programa de ensamblaje de cilios.

Alexander Spektor¹, William Y Tsang, David Khoo

¹Department of Pathology and NYU Cancer Institute, New York University School of Medicine, Smilow Research Center, 522 First Avenue, New York, NY 10016, USA.

|August 28, 2007

Resumen

Los investigadores identificaron Cep97, una proteína que recluta CP110 a los centrosomas. La pérdida de Cep97 o CP110 promueve la formación de cilios primarios, lo que sugiere que colaboran para inhibir la ciliogénesis e impactar en las enfermedades ciliares humanas.

Más Videos Relacionados

Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry

Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry

Published on: December 28, 2021

Generation of Centromere-Associated Protein-E CENP-E-/- Knockout Cell Lines using the CRISPR/Cas9 System

Generation of Centromere-Associated Protein-E CENP-E^-/- Knockout Cell Lines using the CRISPR/Cas9 System

Published on: June 23, 2023

Videos de Experimentos Relacionados

Last Updated: May 13, 2026

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion

Published on: June 25, 2017

Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry

Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry

Published on: December 28, 2021

Generation of Centromere-Associated Protein-E CENP-E-/- Knockout Cell Lines using the CRISPR/Cas9 System

Generation of Centromere-Associated Protein-E CENP-E^-/- Knockout Cell Lines using the CRISPR/Cas9 System

Published on: June 23, 2023

Área de la Ciencia:

Biología celular Biología celular.
Biología Molecular Biología Molecular

Sus antecedentes:

Los centrosomas, cruciales para la división celular, también nuclean el conjunto de cilios.
Los reguladores clave que controlan el destino del centríolo (centrosoma frente a los cilios) son en gran medida desconocidos.

Objetivo del estudio:

Identificar nuevos reguladores de la formación de centrosomas y cilios.
Para aclarar los roles de CP110 y sus socios que interactúan en estos procesos.

Principales métodos:

Purificación de complejos proteicos asociados con CP110.
Estudios de agotamiento y expresión mutante dominante-negativa.
Análisis de la formación del centrosoma, del eje y de los cilios primarios.

Principales resultados:

Se identificó a Cep97 como una nueva proteína que recluta CP110 a los centrosomas.
El agotamiento o la inhibición de Cep97 causa la pérdida de CP110, defectos en el huso y poliploidía.
La pérdida de Cep97 o CP110 promueve la formación de cilios primarios, mientras que la sobreexpresión de CP110 lo inhibe.

Conclusiones:

Cep97 y CP110 funcionan juntos para suprimir la ciliogénesis en las células en crecimiento.
Comprender la regulación de Cep97 y CP110 es crucial para descifrar las enfermedades ciliares humanas como las que afectan a los riñones y las retinas.