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La señalización Wnt posiciona la conectividad neuromuscular inhibiendo la formación de sinapsis en C. elegans
1Department of Biological Sciences and Neurosciences Program, Stanford University, Stanford, CA 94305, USA.
Cell
|August 28, 2007
Resumen
La señalización Wnt restringe la formación de sinapsis en las neuronas de C. elegans. La interrupción de esta vía hace que las sinapsis se formen en los lugares equivocados, revelando cómo las señales de Wnt modelan las conexiones neuronales.
Área de la Ciencia:
- La neurociencia es la neurociencia.
- Biología del desarrollo Biología del desarrollo.
- Biología Molecular Biología Molecular
Sus antecedentes:
- Las conexiones sinápticas son cruciales para la función del sistema nervioso.
- Si bien se conocen los reguladores positivos de la sinaptogénesis, los reguladores negativos son menos conocidos.
- El patrón axonal controla con precisión dónde se forman las sinapsis.
Objetivo del estudio:
- Investigar el papel de los reguladores negativos en el control espacial de la sinaptogénesis.
- Examinar la función de la vía de señalización Wnt en la restricción de la formación de sinapsis en la neurona motora DA9 de Caenorhabditis elegans.
Principales métodos:
- Análisis genético de la formación de sinapsis en las neuronas motoras C. elegans DA9.
- Investigó los componentes de la vía de señalización Wnt: lin-44, egl-20, lin-17/Frizzled y dsh-1/Dishevelled.
- Utilizó la sobreexpresión y el compromiso genético de los componentes de la vía para observar los efectos en la localización presináptica.
Principales resultados:
- El componente de la vía Wnt lin-44 y su receptor lin-17/Frizzled se localizan en un subdominio de axones que normalmente carece de especializaciones presinápticas.
- El compromiso de la vía Wnt (lin-44, egl-20, lin-17/Frizzled, dsh-1/Dishevelled) conduce a la formación de sinapsis ectópicas en este subdominio.
- La sobreexpresión de LIN-44 expande la localización de LIN-17, inhibiendo el ensamblaje presináptico y demostrando la regulación espacial de la sinaptogénesis.
Conclusiones:
- La señalización Wnt, a través de lin-44 y lin-17/Frizzled, restringe espacialmente el ensamblaje presináptico en el axón de la neurona motora DA9.
- Esta vía subdivide el axón en dominios distintos, uno permisivo y otro no permisivo para la formación de sinapsis.
- Las señales morfogenéticas pueden modelar las conexiones sinápticas mediante la creación de dominios axonales espacialmente segregados.
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