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Videos de Conceptos Relacionados

Subcellular Fractionation01:32

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
Turbulent Flow01:24

Turbulent Flow

Turbulent flow is characterized by unpredictable fluctuations in velocity and pressure, which result in a chaotic fluid movement distinct from the orderly patterns of laminar flow. While laminar flow is governed by smooth, parallel layers with minimal mixing, turbulent flow exhibits highly irregular, three-dimensional patterns. This behavior arises due to instabilities in the fluid's velocity profile, and amplifies as the flow velocity increases. Minor disturbances, known as turbulent spots,...
Rapidly Varying Flow01:24

Rapidly Varying Flow

Rapidly varying flow (RVF) in open channels is characterized by abrupt changes in flow depth over a short distance, with the rate of depth change relative to distance often approaching unity. These flows are inherently complex due to their transient and multi-dimensional nature, making exact analysis difficult. However, approximate solutions using simplified models provide valuable insights into their behavior.Key Features of Rapidly Varying FlowRVF is commonly observed in scenarios involving...
Gradually Varying Flow01:29

Gradually Varying Flow

Gradually varying flow (GVF) in open channels describes situations where water depth changes slowly along the channel due to factors like non-uniform bed slope, channel shape variations, or obstructions. This flow type occurs when the depth adjusts gradually to balance gravitational forces, shear forces, and energy requirements, resulting in a low rate of depth change.Characteristics of Gradually Varying FlowGVF is commonly observed in natural streams, rivers, and canals, where flow depth...
Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
Downstream Processing01:29

Downstream Processing

Downstream processing begins once fermentation is complete and involves a series of steps to recover and purify products such as acids, vitamins, antibiotics, or proteins.Cell HarvestingFor example, for intracellular protein-based products, the first step is harvesting the cells. This is typically achieved using centrifugation or filtration to separate the cells from the liquid phase.Cell Disruption for Intracellular ProductsIf the target product is intracellular, the harvested cells must be...

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Video Experimental Relacionado

Updated: Jul 12, 2026

Asymmetrical Flow Field-Flow Fractionation for Sizing of Gold Nanoparticles in Suspension
09:33

Asymmetrical Flow Field-Flow Fractionation for Sizing of Gold Nanoparticles in Suspension

Published on: September 11, 2020

Fraccionamiento del flujo del campo de sedimentación: aplicaciones.

J J Kirkland, W W Yau

    Science (New York, N.Y.)
    |October 8, 1982
    PubMed
    Resumen

    La fraccionamiento de flujo de campo de sedimentación (SFFF) ofrece una separación de alta resolución para las macromoléculas y partículas biológicas. Esta técnica avanzada logra una resolución superior en menos tiempo en comparación con los métodos tradicionales de centrifugación.

    Área de la Ciencia:

    • Química analítica Química analítica es la que
    • La biofísica es la biofísica.
    • Ciencia de los materiales Ciencia de los materiales.

    Sus antecedentes:

    • El fraccionamiento por flujo de campo de sedimentación (SFFF) es una técnica de separación emergente.
    • Los métodos convencionales de centrifugación tienen limitaciones en cuanto a resolución y eficiencia en el tiempo.

    Objetivo del estudio:

    • Para resaltar las capacidades de SFFF para la separación de macromoléculas biológicas, coloides y partículas.
    • Para comparar el rendimiento del SFFF con la centrifugación convencional.

    Principales métodos:

    • Utilizando instrumentación avanzada para el funcionamiento del SFFF.
    • Alcanzando altas velocidades de rotor de hasta 32.000 rpm (aprox. 85.000 g). y el resto de las plantas.

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    Principales resultados:

    • SFFF permite la separación de alta resolución de diversos materiales, incluidos aquellos con pesos moleculares tan bajos como 5 x 10^5.5.
    • El método demuestra una resolución superior en comparación con la centrifugación.
    • SFFF logra estas separaciones en plazos significativamente más cortos.

    Conclusiones:

    • SFFF es un método potente y eficiente para separar una amplia gama de materiales biológicos y de partículas.
    • La técnica ofrece ventajas en resolución y velocidad sobre los métodos convencionales.
    • Los avances en la instrumentación mejoran la aplicabilidad de SFFF para muestras complejas.