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Proofreading01:43

Proofreading

Synthesis of new DNA molecules starts when DNA polymerase links nucleotides together in a sequence that is complementary to the template DNA strand. DNA polymerase has a higher affinity for the correct base to ensure fidelity in DNA replication. The DNA polymerase furthermore proofreads during replication, using an exonuclease domain that cuts off incorrect nucleotides from the nascent DNA strand.Errors during Replication Are Corrected by the DNA Polymerase EnzymeGenomic DNA is synthesized in...
Proofreading01:43

Proofreading

Synthesis of new DNA molecules starts when DNA polymerase links nucleotides together in a sequence that is complementary to the template DNA strand. DNA polymerase has a higher affinity for the correct base to ensure fidelity in DNA replication. The DNA polymerase furthermore proofreads during replication, using an exonuclease domain that cuts off incorrect nucleotides from the nascent DNA strand.Errors during Replication Are Corrected by the DNA Polymerase EnzymeGenomic DNA is synthesized in...
Termination of Translation01:44

Termination of Translation

The large ribosomal subunit has several important structures essential to translation. These include the peptidyl transferase center (PTC) - which is the site where the peptide bond is formed - and a large, internal, water-filled tube through which the nascent polypeptide moves. This latter structure is called the Peptide Exit Tunnel, and it begins at the PTC and spans the body of the large ribosomal subunit. During translation, as the nascent polypeptide chain is synthesized, it passes through...
Termination of Translation01:44

Termination of Translation

The large ribosomal subunit has several important structures essential to translation. These include the peptidyl transferase center (PTC) - which is the site where the peptide bond is formed - and a large, internal, water-filled tube through which the nascent polypeptide moves. This latter structure is called the Peptide Exit Tunnel, and it begins at the PTC and spans the body of the large ribosomal subunit. During translation, as the nascent polypeptide chain is synthesized, it passes through...
Proofreading01:31

Proofreading

Synthesis of new DNA molecules is carried out by the enzyme DNA polymerase, which adds nucleotides on the daughter strand complementary to the template DNA strand. DNA polymerase has a higher affinity to add the correct base and ensures fidelity during DNA replication. Furthermore,  it exhibits proofreading activity during replication, using an exonuclease domain that cuts off incorrect nucleotides from the nascent DNA strand.
Errors During Replication are Corrected by the DNA Polymerase Enzyme
Variance01:15

Variance

The deviations show how spread out the data are about the mean. A positive deviation occurs when the data value exceeds the mean, whereas a negative deviation occurs when the data value is less than the mean. If the deviations are added, the sum is always zero. So one cannot simply add the deviations to get the data spread. By squaring the deviations, the numbers are made positive; thus, their sum will also be positive.The standard deviation measures the spread in the same units as the data.

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Video Experimental Relacionado

Updated: Jul 1, 2026

Molecular Evolution of the Tre Recombinase
12:02

Molecular Evolution of the Tre Recombinase

Published on: May 29, 2008

Fue Erratum Erratum.

    Science (New York, N.Y.)
    |August 7, 1987
    PubMed
    Resumen

    Se emitieron correcciones para un informe sobre los fagos T-even. El estudio encontró que el intrón nrdB ocurre de manera variable, lo que sugiere movilidad dentro de estos genomas de fagos.

    Área de la Ciencia:

    • Biología Molecular Biología Molecular
    • Virología Virología.
    • Genética La genética.

    Sus antecedentes:

    • El informe "La ocurrencia variable del intrón nrdB en los fagos T-even sugiere movilidad de intrones" por Pedersen-Lane y Belfort contenía errores de impresión.
    • La presentación de informes científicos precisos es crucial para la difusión de los hallazgos de la investigación en biología molecular y virología.

    Objetivo del estudio:

    • Para proporcionar oraciones corregidas del informe original.
    • Asegurar una comprensión precisa de la homología de intrones y los métodos experimentales en los fagos T-even.

    Principales métodos:

    • Corrección de frases específicas dentro del informe publicado.
    • Referencia a la hibridación y el análisis de extensión de primeras de dideoxy del ARN pre-mensajero (pre-ARNm).

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    Molecular Evolution of the Tre Recombinase
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  • Aclaración de las descripciones de las sondas de oligonucleótidos utilizadas en las leyendas de figuras.

    • Principales resultados:

    • El intrón td es homólogo en todos los fagos T-igual.
    • La validación experimental incluyó la hibridación y el análisis de primer-extensión.
    • Las sondas de oligonucleótidos se definieron claramente para la reproducibilidad experimental.

    Conclusiones:

    • La información corregida aclara la homología del intrón td en los fagos T-even.
    • Las descripciones precisas de la metodología son esenciales para validar los hallazgos sobre la movilidad intrónica.