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Videos de Conceptos Relacionados

Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Transmission Electron Microscopy01:15

Transmission Electron Microscopy

In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400 keV in...

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Video Experimental Relacionado

Updated: Jul 12, 2026

All-electronic Nanosecond-resolved Scanning Tunneling Microscopy: Facilitating the Investigation of Single Dopant Charge Dynamics
11:33

All-electronic Nanosecond-resolved Scanning Tunneling Microscopy: Facilitating the Investigation of Single Dopant Charge Dynamics

Published on: January 19, 2018

Resolución de picosegundos en microscopía de túnel de barrido.

G Nunes, M R Freeman

    Science (New York, N.Y.)
    |November 12, 1993
    PubMed
    Resumen

    Los investigadores desarrollaron un nuevo método para experimentos de rápida resolución de tiempo utilizando microscopios de túnel de barrido. Esta técnica logra una resolución de tiempo de picosegundos, lo que permite la investigación a escala atómica de los fenómenos dinámicos.

    Área de la Ciencia:

    • Física Física es la física de las cosas.
    • Ciencia de los materiales Ciencia de los materiales.
    • Ciencias de la superficie Ciencias de la superficie.

    Sus antecedentes:

    • La microscopía de túnel de barrido (STM, por sus siglas en inglés) ofrece resolución espacial a escala atómica.
    • Investigar fenómenos dinámicos ultrarrápidos a nanoescala requiere una alta resolución temporal.

    Objetivo del estudio:

    • Desarrollar un método para experimentos de rápida resolución de tiempo utilizando STM.
    • Combinar la resolución espacial del STM con técnicas ópticas ultrarrápidas para estudios dinámicos a nanoescala.

    Principales métodos:

    • Utilizó la no linealidad intrínseca en las características de corriente-voltaje del microscopio de túnel de exploración.
    • Empleó métodos ópticos ultrarrápidos para generar señales transitorias.

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  • Se logró una resolución en escala de tiempo de picosegundos para mediciones experimentales.

    • Principales resultados:

    • Resolvió con éxito señales transitorias generadas ópticamente en escalas de tiempo de picosegundos.
    • Demostró la capacidad de combinar la resolución espacial a escala atómica con una resolución de tiempo ultrarrápida.

    Conclusiones:

    • El método desarrollado proporciona una poderosa herramienta para investigar fenómenos dinámicos a escala atómica.
    • Esta técnica abre nuevas vías para el estudio de procesos transitorios en materiales y superficies con un detalle sin precedentes.