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Un producto genético aroA alterado confiere resistencia al herbicida glifosato
Science (New York, N.Y.)
|July 22, 1983
Resumen
El herbicida glifosato inhibe la 5-enolpiruvilo-3-fosfosfosquímato sintetasa. también conocido como glifosato. Los investigadores identificaron una enzima mutante resistente al glifosato, confirmando su papel en la síntesis de aminoácidos aromáticos y la acción herbicida.
Área de la Ciencia:
- La bioquímica es la bioquímica.
- Biología Molecular Biología Molecular
- Microbiología Microbiología.
Sus antecedentes:
- El glifosato es un herbicida ampliamente utilizado.
- Su mecanismo propuesto consiste en inhibir la vía del shikimato, crucial para la síntesis de aminoácidos aromáticos.
- Esta vía está presente en plantas y microorganismos, pero no en animales.
Objetivo del estudio:
- Para probar la hipótesis de que el glifosato inhibe la 5-enolpiruvilo-3-fosfosfosquímato sintetasa.
- Para aislar y caracterizar mutantes resistentes al glifosato.
Principales métodos:
- Mutagenesis de Salmonella typhimurium mediante el uso de metanesulfonato de etil.
- Selección de la resistencia al glifosato para identificar mutaciones en el gen aroA.
- Clonado del gen mutante aroA y el alelo de tipo salvaje.
- Introducción del gen mutante en Escherichia coli.
Principales resultados:
- Se aisló un mutante de Salmonella typhimurium con un gen aroA que codifica una 5-enolpiruvilo-3-fosfosfosquímato sintetasa resistente al glifosato.
- El gen aroA mutante clonado confería una alta resistencia al glifosato a Escherichia coli.
- Esta resistencia se observó independientemente de la presencia del alelo aroA de tipo salvaje.
Conclusiones:
- El estudio confirma que el glifosato inhibe la 5-enolpiruvilo-3-fosfosfoshikimato sintetasa.
- Las mutaciones en el gen aroA pueden conferir resistencia al glifosato.
- Esto proporciona una base molecular para comprender la resistencia al glifosato en los microorganismos.
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