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Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
Computed Tomography01:10

Computed Tomography

Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
X-ray Imaging01:24

X-ray Imaging

German physicist Wilhelm Röntgen (1845–1923) was experimenting with electrical current when he discovered that a mysterious and invisible "ray" would pass through his flesh but leave an outline of his bones on a screen coated with a metal compound. In 1895, Röntgen made the first durable record of the internal parts of a living human: an "X-ray" image (as it came to be called) of his wife’s hand. Scientists worldwide quickly began their own experiments with X-rays, and by 1900, X-ray was widely...
Imaging Studies I: CT and MRI01:14

Imaging Studies I: CT and MRI

Introduction: MRI and CT scans are crucial advancements in medical imaging techniques, playing a vital role in diagnosing conditions related to the gastrointestinal (GI) system. Each scan serves distinct purposes, targets specific areas, and requires unique nursing duties.
Description of the Procedures
Computed Tomography (CT) scan:
Computed Tomography (CT) scans use X-ray technology to generate detailed images of bones, organs, and tissues. During the scan, the patient lies on a moving table...

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Video Experimental Relacionado

Updated: Jul 12, 2026

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages
08:46

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

Published on: April 13, 2016

Microtomografía tridimensional de rayos X con rayos X.

B P Flannery, H W Deckman, W G Roberge

    Science (New York, N.Y.)
    |September 18, 1987
    PubMed
    Resumen

    La microtomografía de rayos X ofrece un nuevo método no destructivo para crear mapas detallados en 3D de la composición del material. Esta técnica logra una alta precisión y resolución, lo que permite un análisis elemental preciso para muestras pequeñas.

    Área de la Ciencia:

    • Ciencia de los materiales Ciencia de los materiales.
    • Física Física es la física de las cosas.
    • Tecnología de imágenes de tecnología de imagen.

    Sus antecedentes:

    • La microtomografía de rayos X es una técnica poderosa para el análisis no destructivo.
    • El mapeo 3D preciso de las propiedades de los materiales es crucial en varios campos científicos.

    Objetivo del estudio:

    • Introducir y describir una nueva técnica de microtomografía de rayos X.
    • Para demostrar su capacidad para generar mapas 3D de alta precisión y alta resolución.
    • Para mostrar el mapeo elemental utilizando fuentes de sincrotrón.

    Principales métodos:

    • Utilizó la microtomografía de rayos X para imágenes 3D no destructivas.
    • Empleado sincrotrón fuentes de rayos X para el mapeo elemental con resolución espacial.

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    Last Updated: Jul 12, 2026

    Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages
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    Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

    Published on: May 27, 2008

  • Desarrolló algoritmos de alta velocidad para la reconstrucción tomográfica.
  • Integrado un detector de rayos X de imagen de alta resolución.

    • Principales resultados:

    • Logró aproximadamente un 1 por ciento de precisión en el mapeo del coeficiente de atenuación de rayos X.
    • Alcanzó una resolución espacial cercana a 1 micrómetro.
    • Produjo con éxito mapas elementales con resolución espacial.
    • Se presentaron imágenes tomográficas que demuestran el rendimiento con sincrotrón y fuentes de laboratorio.

    Conclusiones:

    • La nueva técnica de microtomografía de rayos X proporciona una caracterización de material 3D precisa y de alta resolución.
    • Permite el análisis elemental detallado, en particular con la radiación de sincrotrón.
    • El sistema es efectivo tanto para aplicaciones basadas en sincrotrones como para aplicaciones basadas en laboratorios.