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Videos de Conceptos Relacionados

Atomic Fluorescence Spectroscopy01:29

Atomic Fluorescence Spectroscopy

Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which are...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
Mass Spectrometers01:16

Mass Spectrometers

This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
Atomic Absorption Spectroscopy: Atomization Methods01:25

Atomic Absorption Spectroscopy: Atomization Methods

Atomic Absorption Spectroscopy (AAS) atomizes samples through flame atomization or electrothermal atomization. Flame atomization typically involves a nebulizer and spray chamber assembly to combine the sample with a fuel–oxidant mixture, creating a fine aerosol mist that enters a burner. Typically, the fuel and oxidant are combined in an approximately stoichiometric ratio. However, for atoms that are easily oxidized, a fuel-rich mixture may be more advantageous. Only about 5% of the aerosol...
Mass Analyzers: Common Types01:19

Mass Analyzers: Common Types

The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...

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Video Experimental Relacionado

Updated: Jul 11, 2026

Measurement of Ultrafast Vibrational Coherences in Polyatomic Radical Cations with Strong-Field Adiabatic Ionization
08:22

Measurement of Ultrafast Vibrational Coherences in Polyatomic Radical Cations with Strong-Field Adiabatic Ionization

Published on: August 6, 2018

Espectrometría de masas de bombardeo atómico rápido por bombardeo atómico rápido.

K L Rinehart

    Science (New York, N.Y.)
    |October 15, 1982
    PubMed
    Resumen

    La espectrometría de masas de bombardeo atómico rápido (FAB MS) es una técnica poderosa para analizar moléculas grandes. Efectivamente ioniza y detecta racimos y péptidos inorgánicos, ayudando en la determinación estructural.

    Área de la Ciencia:

    • Química Analítica La Química Analítica es la
    • La espectrometría de la espectrometría.

    Sus antecedentes:

    • La espectrometría de masas de bombardeo atómico rápido (FAB MS) surgió como una herramienta analítica significativa en 1981.
    • Permite la ionización de muestras en estado condensado, típicamente dentro de una matriz de glicerol.

    Objetivo del estudio:

    • Para resaltar las capacidades de FAB MS como un método de aclaración estructural.
    • Para mostrar su aplicación en el análisis de diversas entidades químicas.

    Principales métodos:

    • Las muestras son bombardeadas con átomos de xenón o argón de alta energía (500010.000 eV).
    • Este proceso genera iones secundarios (positivos y negativos) pulverizados desde la superficie de la muestra.

    Principales resultados:

    Más Videos Relacionados

    Analyzing Large Protein Complexes by Structural Mass Spectrometry
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    Analyzing Large Protein Complexes by Structural Mass Spectrometry

    Published on: June 19, 2010

    PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis
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    PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis

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    Last Updated: Jul 11, 2026

    Measurement of Ultrafast Vibrational Coherences in Polyatomic Radical Cations with Strong-Field Adiabatic Ionization
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    Measurement of Ultrafast Vibrational Coherences in Polyatomic Radical Cations with Strong-Field Adiabatic Ionization

    Published on: August 6, 2018

    Analyzing Large Protein Complexes by Structural Mass Spectrometry
    15:35

    Analyzing Large Protein Complexes by Structural Mass Spectrometry

    Published on: June 19, 2010

    PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis
    08:43

    PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis

    Published on: May 11, 2017

    • FAB MS detectó con éxito racimos de iones inorgánicos de hasta una masa de 25.800.
    • Se detectaron péptidos biológicamente activos hasta una masa de 5700.
    • Se obtuvieron iones moleculares para compuestos polares y labiles como los glucosfingolipidos y los antibióticos poliénicos.

    Conclusiones:

    • FAB MS es altamente eficaz para determinar la estructura de moléculas complejas.
    • La técnica es particularmente valiosa para la secuenciación de aminoácidos en polipéptidos.
    • Proporciona información molecular para desafiar tipos de muestras que antes eran difíciles de analizar.