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Atomic Absorption Spectroscopy: Radiation and Light Sources01:13

Atomic Absorption Spectroscopy: Radiation and Light Sources

Atomic absorption spectroscopy (AAS) relies on the Beer-Lambert law, which requires that the radiation source emits a narrow range of wavelengths to match the absorption characteristics of the analyte atom. The primary criteria for choosing an appropriate radiation source in AAS is to provide a precise and intense emission at specific wavelengths that will allow accurate detection of the analyte.
Two common narrow-range 'line' sources used in AAS are hollow-cathode lamps (HCLs) and...
X-ray Imaging01:24

X-ray Imaging

German physicist Wilhelm Röntgen (1845–1923) was experimenting with electrical current when he discovered that a mysterious and invisible "ray" would pass through his flesh but leave an outline of his bones on a screen coated with a metal compound. In 1895, Röntgen made the first durable record of the internal parts of a living human: an "X-ray" image (as it came to be called) of his wife’s hand. Scientists worldwide quickly began their own experiments with X-rays, and by 1900, X-ray was widely...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
Photoluminescence: Applications01:14

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
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X-ray Diffraction of Biological Samples

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Video Experimental Relacionado

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Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages
08:46

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

Published on: April 13, 2016

Ver con una nueva luz: la radiación del sincrotrón.

R E Watson, M L Perlman

    Science (New York, N.Y.)
    |March 24, 1978
    PubMed
    Resumen

    La luz sincrotrón, con sus propiedades únicas, ofrece aplicaciones versátiles en investigación y tecnología. Su amplio espectro permite técnicas avanzadas en espectroscopia, microscopia y ciencia de los materiales, impulsando la innovación.

    Área de la Ciencia:

    • Utiliza los principios de la física y la ciencia de los materiales.
    • Aprovecha técnicas avanzadas de espectroscopia, microscopía y dispersión.

    Sus antecedentes:

    • La luz sincrotrón posee propiedades únicas que impulsan el aumento de la investigación y las aplicaciones tecnológicas.
    • El amplio espectro de la radiación de sincrotrón abarca desde el ultravioleta hasta las regiones suaves de rayos X.

    Objetivo del estudio:

    • Describir las características de la radiación sincrotrón y sus fuentes.
    • Presentar investigaciones y aplicaciones típicas que muestran la versatilidad de las fuentes de sincrotrones.

    Principales métodos:

    • Exploración de espectroscopias atómicas, moleculares y de estado sólido en la región ultravioleta.
    • Aplicación de rayos X blandos para la espectroscopia, la litografía, la microscopia y la topografía.

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  • Utilizando las propiedades de dispersión en longitudes de onda más cortas para sondear la estructura del material.

    • Principales resultados:

    • Demuestra diversas aplicaciones de la luz del sincrotrón en diversas disciplinas científicas.
    • Destaca la adaptabilidad de la radiación de sincrotrón para investigaciones complejas.

    Conclusiones:

    • La luz sincrotrón es una herramienta poderosa y versátil para el descubrimiento científico y el avance tecnológico.
    • Sus propiedades únicas facilitan una amplia gama de metodologías de investigación de vanguardia.