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Arquitectura funcional y evolución de los elementos transcripcionales que impulsan la coexpresión génica
Christopher D Brown1, David S Johnson, Arend Sidow
1Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA.
Science (New York, N.Y.)
|September 18, 2007
Resumen
La investigación de los elementos cis-reguladores que controlan la coexpresión génica en Ciona reveló que si bien la actividad de los motivos individuales varía, los motivos ortólogos se conservan. Las restricciones evolutivas dan forma a estos elementos reguladores, impactando la regulación de los genes.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- La genómica es la genómica.
- Biología evolutiva Biología evolutiva.
Sus antecedentes:
- La coexpresión transcripcional de los productos genéticos en interacción es crucial para los procesos moleculares complejos.
- La función y la evolución de los elementos cis-reguladores que orquestan la coexpresión no se comprenden bien.
Objetivo del estudio:
- Investigar la actividad y las restricciones evolutivas de los elementos cis-reguladores y sus motivos constituyentes que impulsan la coexpresión de genes musculares en Ciona.
Principales métodos:
- Mutagenesis de 19 elementos reguladores que controlan la coexpresión del gen del músculo Ciona.
- Estimación cuantitativa de la actividad cis-reguladora de 77 motivos constituyentes.
- Análisis comparativo de motivos ortólogos en diferentes genes corregidos.
Principales resultados:
- La actividad de los motivos individuales muestra una amplia variación dentro y entre los elementos y tipos de motivos.
- La actividad del motivo ortólogo está fuertemente restringida a través del tiempo evolutivo.
- La disposición, el tipo y la actividad de los motivos varían significativamente entre los elementos que regulan diferentes genes coexpresados.
Conclusiones:
- Las reglas sintácticas que rigen la función cis-regulatoria son flexibles, pero se convierten en restricciones evolutivas una vez establecidas.
- Comprender la dinámica de los motivos es clave para descifrar la compleja regulación y evolución de los genes.
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