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Analysis of Histone Antibody Specificity with Peptide Microarrays
Published on: August 1, 2017
JMJD6 es una histona arginina desmetilasa
Bingsheng Chang1, Yue Chen, Yingming Zhao
1Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-9038, USA.
Science (New York, N.Y.)
|October 20, 2007
Resumen
La proteína 6 que contiene el dominio Jumonji (JMJD6) desmetila los residuos de histona H3 y H4 de arginina, revirtiendo las modificaciones regulatorias. Este descubrimiento puede explicar los defectos de desarrollo en los ratones knockout JMJD6.
Área de la Ciencia:
- La bioquímica es la bioquímica.
- Biología Molecular Biología Molecular
- La epigenética es la epigenética.
Sus antecedentes:
- La metilación de la arginina regula diversas funciones celulares.
- La metilación de la histona arginina (mono y dimetilación) tiene un impacto en la remodelación de la cromatina y la expresión génica.
- Ninguna enzima conocida invierte la metilación de la histona arginina.
Objetivo del estudio:
- Para identificar una enzima capaz de revertir la metilación de la histona arginina.
- Para caracterizar la actividad enzimática del dominio Jumonji que contiene la proteína 6 (JMJD6).
Principales métodos:
- Se emplearon ensayos bioquímicos para probar la actividad enzimática de JMJD6.
- Se utilizaron ensayos basados en células para confirmar la función de JMJD6 in vivo.
- JMJD6 fue identificado como una dioxigenasa dependiente de hierro y 2-oxoglutarato que contiene JmjC.
Principales resultados:
- Se demostró que JMJD6 desmetila la histona H3 en la arginina 2 (H3R2).
- Se demostró que JMJD6 desmetila la histona H4 en la arginina 3 (H4R3).
- Estas actividades de desmetilación se confirmaron en ensayos bioquímicos y basados en células.
Conclusiones:
- JMJD6 funciona como una histona arginina desmetilasa.
- La actividad de JMJD6 proporciona un mecanismo para revertir la metilación de la histona arginina.
- Estos hallazgos ofrecen una posible explicación para los defectos de desarrollo observados en ratones knockout JMJD6.
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