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El reconocimiento de patógenos vegetales mediado por la activación del promotor del gen de resistencia Bs3 del
Patrick Römer1, Simone Hahn, Tina Jordan
1Institute of Biology, Department of Genetics, Martin-Luther-University Halle-Wittenberg, D-06099 Halle (Saale), Germany.
Resumen
Las proteínas de resistencia a las enfermedades de las plantas reconocen a los efectores de patógenos. El gen Bs3 de la pimienta.
Área de la Ciencia:
- Patología vegetal patología vegetal.
- Biología molecular La biología molecular.
- Genética La genética.
Sus antecedentes:
- Las proteínas de resistencia a las enfermedades de las plantas (R) son cruciales para la inmunidad de las plantas, reconociendo proteínas de avirulencia de patógenos específicos (Avr).
- El gen Bs3 de la pimienta y sus alelos confieren resistencia contra Xanthomonas campestris pv. vesicatoria (Xcv). también conocido como vesicatoria.
Objetivo del estudio:
- Aclarar el mecanismo de reconocimiento entre las proteínas de resistencia Bs3 de la pimienta y las proteínas efectoras Xcv tipo III AvrBs3 y AvrBs3Deltarep16.
- Investigar el papel de las regiones promotoras en la especificidad del reconocimiento de patógenos mediados por el gen R.
Principales métodos:
- Análisis de alelos de genes Bs3 y Bs3-E y sus regiones promotoras.
- Investigando la activación transcripcional de Bs3 y Bs3-E por AvrBs3 y AvrBs3Deltarep16.
- Determinación de los sitios de unión de AvrBs3/AvrBs3Deltarep16 dentro de las regiones promotoras Bs3/Bs3-E.
Principales resultados:
- La pimienta Bs3 y su alelo Bs3-E codifican para las flavin monooxigenasas y son activadas transcripcionalmente por proteínas afines Avr.
- La especificidad de reconocimiento está determinada por la interacción de AvrBs3 o AvrBs3Deltarep16 con secuencias específicas de ADN en los promotores Bs3 y Bs3-E.
- Las proteínas Avr se unen y activan al promotor de su correspondiente gen R.
Conclusiones:
- Se propone un nuevo mecanismo de reconocimiento de patógenos vegetales en el que las proteínas Avr se unen y activan directamente al promotor del gen R. congénito.
- Esta interacción dicta la especificidad de la resistencia a las enfermedades de las plantas.
- Los hallazgos proporcionan información sobre la base molecular de la inmunidad de las plantas y la función del gen R.
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