Síntesis de nucleótidos de 5-fluoropirimidina como sensitivas sondas de RMN de la estructura del ARN

Mirko Hennig1, Lincoln G Scott, Edit Sperling

  • 1Department of Biochemistry and Molecular Biology, Medical University of South Carolina, 173 Ashley Avenue, P.O. Box 250509, Charleston, South Carolina 29425, USA. hennig@musc.edu

Resumen

Nuevos métodos enzimáticos permiten la síntesis de trifosfato de 5-fluorouridina (5F-UTP) y trifosfato de 5-fluorocitidina (5F-CTP) para el ARN de etiquetado 19F. Esto facilita los estudios estructurales y biofísicos de las moléculas de ARN.

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