Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
The Mitotic Spindle02:27

The Mitotic Spindle

The mitotic spindle—or spindle apparatus—is a eukaryotic, cytoskeletal structure made up of long protein fibers called microtubules. Formed during cell division, the spindle separates sister chromatids and moves them to opposite ends of a parental cell, where the now individual chromosomes are distributed to two daughter cell nuclei.
The bipolar configuration of the mitotic spindle facilitates chromosomal segregation, preparing the cell for division. One mechanism that ensures bipolar mitotic...
The Mitotic Spindle02:27

The Mitotic Spindle

The mitotic spindle—or spindle apparatus—is a eukaryotic, cytoskeletal structure made up of long protein fibers called microtubules. Formed during cell division, the spindle separates sister chromatids and moves them to opposite ends of a parental cell, where the now individual chromosomes are distributed to two daughter cell nuclei.
The bipolar configuration of the mitotic spindle facilitates chromosomal segregation, preparing the cell for division. One mechanism that ensures bipolar mitotic...
Spindle Assembly02:50

Spindle Assembly

Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a microtubule array...
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Author Correction: Mechanisms of active wetting and fluidification in epithelial cell collectives.

Nature materials·2026
Same author

Molecular anatomy of PLK1 master docking motifs.

Nature communications·2026
Same author

Molecular basis of cooperative assembly of the Ndc80-Ska kinetochore complex on microtubules.

bioRxiv : the preprint server for biology·2026
Same author

Chromosomal instability promotes cell migration and invasion via EFEMP1 secretion into extracellular vesicles.

The EMBO journal·2026
Same author

Mechanisms of active wetting and fluidification in epithelial cell collectives.

Nature materials·2026
Same author

A multifaceted kinase keeps molecular motors in place for faithful cell division.

Cell reports·2026

Video Experimental Relacionado

Updated: Jul 10, 2026

Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes
10:09

Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes

Published on: September 13, 2022

El dimero conformacional Mad2: estructura e implicaciones para el punto de control de ensamblaje del husillo.

Marina Mapelli1, Lucia Massimiliano, Stefano Santaguida

  • 1Department of Experimental Oncology, European Institute of Oncology, Via Adamello 16, I-20139, Milan, Italy. marina.mapelli@ifom-ieo-campus.it

Cell
|November 21, 2007
PubMed
Resumen

La proteína Mad2 regula la división celular mediante la formación de un único dímero. Esta estructura cristalina revela cómo los conformadores Mad2 abiertos y cerrados se unen selectivamente, lo cual es crucial para prevenir errores cromosómicos durante la mitosis.

Más Videos Relacionados

Studying Mitotic Checkpoint by Illustrating Dynamic Kinetochore Protein Behavior and Chromosome Motion in Living Drosophila Syncytial Embryos
13:59

Studying Mitotic Checkpoint by Illustrating Dynamic Kinetochore Protein Behavior and Chromosome Motion in Living Drosophila Syncytial Embryos

Published on: June 14, 2012

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

Videos de Experimentos Relacionados

Last Updated: Jul 10, 2026

Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes
10:09

Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes

Published on: September 13, 2022

Studying Mitotic Checkpoint by Illustrating Dynamic Kinetochore Protein Behavior and Chromosome Motion in Living Drosophila Syncytial Embryos
13:59

Studying Mitotic Checkpoint by Illustrating Dynamic Kinetochore Protein Behavior and Chromosome Motion in Living Drosophila Syncytial Embryos

Published on: June 14, 2012

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

Área de la Ciencia:

  • Biología celular Biología celular.
  • Biología Molecular Biología Molecular
  • Biología Estructural Biología estructural.

Sus antecedentes:

  • La proteína Mad2 es esencial para el punto de control de ensamblaje del husillo, previniendo errores en la segregación cromosómica durante la mitosis.
  • Mad2 exhibe dos conformaciones distintas (abierta y cerrada) y forma interacciones específicas con Mad1 y Cdc20.
  • Un dímero conformacional de Mad2 abierto (O-Mad2) y cerrado (C-Mad2) es vital para la función del punto de control del husillo en todas las especies.

Objetivo del estudio:

  • Para determinar la estructura cristalina de las dimensiones conformacionales O-Mad2-C-Mad2.
  • Aclarar la base estructural para la dimerización selectiva de los conformeros O-Mad2 y C-Mad2.
  • Comprender el papel de los cambios conformacionales de Mad2 en la regulación del punto de control del husillo.

Principales métodos:

  • Cristalografía de rayos X para obtener la estructura del dimero conformacional O-Mad2-C-Mad2.
  • Análisis estructural para identificar la interfaz de dimerización y los residuos clave.
  • Correlación de los hallazgos estructurales con los cambios topológicos y la función de Mad2.

Principales resultados:

  • La estructura cristalina revela una interfaz asimétrica responsable de la dimerización selectiva de O-Mad2 y C-Mad2.
  • Identificación de residuos hidrofóbicos enterrados cuya reorganización está vinculada a la transición topológica de Mad2.
  • La estructura del dímero soporta un modelo catalítico para la función Mad2 en el punto de control del husillo.

Conclusiones:

  • La estructura de dímeros O-Mad2-C-Mad2 proporciona una visión crítica de los mecanismos de punto de control de ensamblaje de husillo.
  • Los datos estructurales explican la interacción selectiva entre los conformadores Mad2, esenciales para la fidelidad en los puntos de control.
  • Los hallazgos apoyan un modelo en el que C-Mad2 actúa como una plantilla para facilitar la unión de O-Mad2 a Cdc20, asegurando una segregación cromosómica precisa.