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Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures
Published on: June 26, 2020
Invasión de la hebra de secuencia mixta B-ADN por el ácido nucleico gamma-péptido (gamma-PNA) ligado a la acridina
Srinivas Rapireddy1, Gaofei He, Subhadeep Roy
1Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213, USA.
Journal of the American Chemical Society
|November 22, 2007
Resumen
Los ácidos nucleicos gamma-peptídicos (gamma-PNAs) preorganizados conformalmente con una porción de acridina pueden invadir la secuencia mixta B-DNA. Este avance permite el reconocimiento específico de la secuencia de ADN de doble cadena utilizando los principios de Watson-Crick de emparejamiento de bases.
Área de la Ciencia:
- Química sintética es la química sintética.
- Biología molecular La biología molecular.
- Biotecnología La biotecnología es la biotecnología.
Sus antecedentes:
- El ácido nucleico peptídico (PNA) imita el ADN / ARN y se une a los objetivos a través del emparejamiento Watson-Crick.
- Los actuales PNA luchan por unirse al ADN-B de doble cadena de secuencia mixta debido a una energía de unión insuficiente.
Objetivo del estudio:
- Desarrollar una variante PNA capaz de reconocimiento específico de secuencia de secuencia mixta B-DNA.
- Para demostrar la invasión del ADN-B de doble cadena por un PNA modificado.
Principales métodos:
- Utilizado ácido nucleico preorganizado conformacionalmente de péptido gamma (gamma-PNA).
- Un grupo de acridina está vinculado covalentemente al extremo C del gamma-PNA.
- Se ha investigado la unión específica de la secuencia al ADN-B de secuencia mixta.
Principales resultados:
- El gamma-PNA preorganizado conformalmente con una porción de acridina invadió con éxito el ADN-B de secuencia mixta.
- El reconocimiento se produjo a través del emparejamiento directo de bases Watson-Crick.
- Se ha demostrado la unión específica de la secuencia a los objetivos de ADN de doble cadena.
Conclusiones:
- Los gamma-PNA modificados pueden superar las limitaciones del PNA estándar para el reconocimiento de ADN de doble cadena.
- Los principios establecidos de reconocimiento de ADN / ARN de una sola hebra son aplicables al ADN B de doble hebra que utiliza estos PNA modificados.
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