Video Experimental Relacionado
Updated: Jul 9, 2026
07:23
Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
Published on: June 15, 2016
Cambiar de la represión a la activación: los microARN pueden regular al alza la traducción
Shobha Vasudevan1, Yingchun Tong, Joan A Steitz
1Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University School of Medicine, Boyer Center for Molecular Medicine, 295 Congress Avenue, New Haven, CT 06536, USA.
Science (New York, N.Y.)
|December 1, 2007
Resumen
Los microARN (miRNA) pueden activar la traducción génica durante la detención del ciclo celular al unirse a los elementos ricos en UA (AREs) en el ARN mensajero (ARNm). Esta función de activación de las micro-ribonucleoproteínas (miRNP) parece oscilar con la proliferación celular.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Regulación genética Reglamento genético.
- El control post-transcripcional de control después de la transcripción.
Sus antecedentes:
- Los elementos ricos en UA (AREs) y los sitios objetivo de microARN en el ARNm 3'UTR regulan la expresión génica post-transcripcionalmente.
- Durante la detención del ciclo celular, las ERA en el ARNm del factor de necrosis tumoral alfa (TNFalpha) reclutan Argonauta (AGO) y la proteína 1 (FXR1) relacionada con el retraso mental del X frágil, formando micro-ribonucleoproteínas (miRNP).
Objetivo del estudio:
- Investigar el papel del microARN miR369-3 en la dirección de la asociación de proteínas con AREs para la activación de la traducción.
- Para determinar si otros microARN exhiben patrones similares de regulación de la traducción durante la detención y la proliferación del ciclo celular.
Principales métodos:
- Se investigó la interacción de miR369-3 con ERA y proteínas asociadas (AGO, FXR1).
- Se analizó la regulación de la traducción de los ARNm diana por Let-7 y miRcxcr4 durante la detención y proliferación del ciclo celular.
Principales resultados:
- El microARN humano miR369-3 dirige el reclutamiento de AGO y FXR1 a las AREs, activando la traducción del ARNm.
- Let-7 y miRcxcr4 regulan al alza la traducción del ARNm objetivo al detenerse el ciclo celular, pero lo reprimen en las células proliferantes.
- La activación de la traducción por los miRNP es una función común que se observa durante la detención del ciclo celular.
Conclusiones:
- La activación de la traducción mediada por microARN es un mecanismo significativo durante la detención del ciclo celular.
- La regulación de la traducción mediada por microARN-ribonucleoproteína (miRNP) exhibe un patrón oscilante entre la represión y la activación a lo largo del ciclo celular.
Videos de Conceptos Relacionados
01:22MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
01:22MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
01:22MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
01:23Regulation of Expression at Multiple Steps
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...
02:04Co-activators and Co-repressors
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
02:04Co-activators and Co-repressors
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...