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Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay
Published on: October 20, 2018
El origen de las interacciones proteicas y la alostería en la colocalización
John Kuriyan1, David Eisenberg
1Howard Hughes Medical Institute, California Institute for Quantitative Biosciences, Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA.
Nature
|December 14, 2007
Resumen
Las redes de proteínas celulares surgen de las leyes físicas básicas y la selección natural. La colocalización de proteínas amplifica los efectos de la mutación, impulsando la evolución de las interacciones complejas y la regulación alostérica.
Área de la Ciencia:
- La bioquímica es la bioquímica.
- Biología Molecular Biología Molecular
- Biología evolutiva Biología evolutiva.
Sus antecedentes:
- Las redes de proteínas reguladas son esenciales para la función celular.
- Comprender el origen de estas complejas redes es una cuestión fundamental en biología.
Objetivo del estudio:
- Explicar los principios fundamentales que impulsan el origen de las redes de proteínas reguladas.
- Para aclarar el papel de las leyes físicas y la selección natural en la interacción de las proteínas en la evolución.
Principales métodos:
- El estudio es principalmente teórico, basado en principios establecidos de la física y las interacciones moleculares.
- Aplica la ley de acción de la masa y el concepto de colocalización molecular a las interacciones de las proteínas.
Principales resultados:
- La colocalización aumenta significativamente las concentraciones locales, amplificando el impacto de las mutaciones.
- Esta amplificación, bajo la selección natural, impulsa la evolución de las interacciones proteína-proteína y la regulación alostérica.
- Las proteínas homólogas pueden desarrollar distintos mecanismos alostéricos.
Conclusiones:
- La complejidad y regulación observadas en las redes de proteínas son resultados predecibles de la selección natural que actúa sobre las leyes físicas.
- Las leyes físicas que rigen las interacciones moleculares son los motores fundamentales para la evolución de los sistemas reguladores celulares.
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