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La transcripción del ARN polimerasa II es fosforilada en el residuo serina-7 de la CTD

Rob D Chapman¹, Martin Heidemann, Thomas K Albert

¹Institute of Clinical Molecular Biology and Tumour Genetics, GSF-Research Center of Environment and Health, Munich Center for Integrated Protein Science (CiPSM), Marchioninistrasse 25, 81377 Munich, Germany.

Science (New York, N.Y.)

|December 15, 2007

Resumen

La ARN polimerasa II también se encuentra presente.

Área de la Ciencia:

Biología Molecular Biología Molecular
Expresión génica de la expresión génica.
La bioquímica es la bioquímica.

Sus antecedentes:

El dominio de repetición carboxilo-terminal (CTD) de la ARN polimerasa II es crucial para la transcripción.
La fosforilación de CTD, particularmente en serina-2 y serina-5, regula la transcripción y el reclutamiento de factores de procesamiento de ARN.
El papel de otros sitios de fosforilación de CTD, como la serina-7, sigue siendo menos entendido.

Objetivo del estudio:

Para investigar el estado de fosforilación de la serina-7 dentro de la ARN polimerasa II CTD.
Para determinar las implicaciones funcionales de la fosforilación de la serina-7 y su ubicación dentro de las repeticiones de CTD.
Explorar la relación entre los patrones de fosforilación de la serina-7 y la expresión génica óptima.

Principales métodos:

Se utilizaron anticuerpos monoclonales para detectar la fosforilación de la serina-7 en los genes transcritos.
Se analizaron los patrones de fosforilación de CTD en relación con el número y la posición de las repeticiones de consenso.
Perfiles de fosforilación de CTD correlacionados con los niveles de expresión génica.

Principales resultados:

Se identificó la fosforilación de la serina-7 en las CTD de la ARN polimerasa II transcrita.
Se observó que la fosforilación de serina-7 está ausente en CTDs con menos de 20 repeticiones de consenso.
Se demostró que la ubicación de la sustitución de serina-7 influye en distintos patrones de fosforilación, lo que sugiere una heterogeneidad funcional dentro del CTD.
Se encontró que la restricción de los epítopos de serina-7 a la región proximal izquierda limita los patrones de fosforilación de la DTC.

Conclusiones:

La fosforilación de la serina-7 es una nueva modificación de la ARN polimerasa II CTD.
La posición de los sitios de fosforilación de serina-7 dentro del CTD es crítica para regular los patrones de fosforilación.
La expresión génica óptima depende de la restricción de los epítopos de serina-7 a regiones específicas de CTD.

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