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La NADP regula el sistema de inducción GAL de la levadura.
P Rajesh Kumar1, Yao Yu, Rolf Sternglanz
1Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY11724, USA.
Science (New York, N.Y.)
|February 23, 2008
Resumen
El fosfato de nicotinamida adenina dinucleótido (NADP) es un factor clave en la regulación de los genes del metabolismo de la galactosa en las levaduras. Este dinucleótido media la interacción entre Gal4p y Gal80p, lo que explica la rápida inducción de genes.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Genética de la levadura Genética de la levadura
- Reglamento genético Reglamento genético.
Sus antecedentes:
- La regulación transcripcional del metabolismo de la galactosa en Saccharomyces cerevisiae incluye Gal4p, Gal80p y Gal3p.
- El modelo existente de inducción a través del secuestro citoplasmático de Gal80p por Gal3p no explica completamente la respuesta rápida observada.
- Se sospechaba que faltaba un factor en el modelo actual de regulación transcripcional.
Objetivo del estudio:
- Para dilucidar el mecanismo molecular subyacente a la rápida inducción transcripcional de genes que metabolizan la galactosa en la levadura.
- Para identificar el factor faltante involucrado en la interacción entre Gal4p y Gal80p.
- Para caracterizar el papel de cualquier factor identificado en el complejo regulatorio Gal4p-Gal80p.
Principales métodos:
- Análisis estructural de la interacción Gal80p-Gal4p utilizando cristalografía de rayos X.
- Pruebas bioquímicas para investigar las interacciones moleculares.
- Experimentos in vivo en Saccharomyces cerevisiae para validar los hallazgos.
Principales resultados:
- La estructura cristalina de Gal80p unido a un péptido Gal4p reveló un dinucleótido asociado.
- Los estudios bioquímicos y in vivo identificaron el fosfato de nicotinamida adenina dinucleótido (NADP) como este dinucleótido mediador.
- Se demostró que la NADP juega un papel crucial en el evento de inducción inicial de la regulación de la galactosa.
Conclusiones:
- El fosfato de nicotinamida adenina dinucleótido (NADP) es un componente crítico en la regulación transcripcional del metabolismo de la galactosa en la levadura.
- La NADP media la interacción entre el activador transcripcional Gal4p y el represor Gal80p.
- El descubrimiento del papel de la NADP proporciona una comprensión más completa del mecanismo de inducción rápida para los genes inducibles a la galactosa.
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