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Una ciclina de tipo B de levadura de fisión que funciona temprano en el ciclo celular
A Bueno1, H Richardson, S I Reed
1Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, California 92037.
Cell
|July 12, 1991
Resumen
Un gen de levadura de fisión recién descubierto, cig1+, codifica una nueva ciclina de tipo B. Esta ciclina regula la transición de fase G1 a S, distinta de las proteínas del ciclo celular previamente conocidas.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología Molecular Biología Molecular
- Genética La genética.
Sus antecedentes:
- Las ciclinas son reguladores clave del ciclo celular.
- Las ciclinas de tipo B suelen estar involucradas en la progresión mitótica.
- La función de cig1+ en la levadura de fisión era previamente desconocida.
Objetivo del estudio:
- Clonar y caracterizar el gen de la levadura de fisión cig1+.
- Para determinar el papel de la proteína cig1+ en el ciclo celular.
- Para investigar la distinción funcional de cig1+ en comparación con otras ciclinas.
Principales métodos:
- Clonación de genes y caracterización del gen cig1+.
- Estudios de eliminación y sobreexpresión en levaduras de fisión.
- Análisis de la progresión del ciclo celular y la viabilidad.
Principales resultados:
- El gen cig1+ de la levadura de fisión codifica una proteína de 48 kDa con similitud a las ciclinas de tipo B.
- La eliminación de cig1+ causa un retraso significativo en la progresión de la fase G1 a la fase S.
- La sobreexpresión constitutiva de cig1+ es letal y detiene las células en G1.
- cig1+ no rescata a S. cerevisiae que carece de las ciclinas CLN.
Conclusiones:
- cig1+ representa una nueva clase de ciclina tipo B.
- Esta ciclina juega un papel crítico en la regulación de la transición de fase G1 a S.
- cig1+ es funcionalmente distinto de las conocidas ciclinas mitóticas y G1.
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