Jove
Visualize
Contáctanos

Videos de Conceptos Relacionados

Nucleotide Excision Repair01:38

Nucleotide Excision Repair

DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview
Overview of DNA Repair02:25

Overview of DNA Repair

In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
Proteins: From Genes to Degradation02:11

Proteins: From Genes to Degradation

Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick.  Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
Transcription is the synthesis of RNA molecules by RNA...
Proteins: From Genes to Degradation02:11

Proteins: From Genes to Degradation

Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick.  Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
Transcription is the synthesis of RNA molecules by RNA...
Protein Denaturation01:28

Protein Denaturation

The function of proteins depends on their native three-dimensional structure, which is dictated by the amino acid sequence of the specific protein. Folding of the polypeptide chain takes place under specific conditions that energetically favor the folded conformation. In contrast, protein denaturation occurs spontaneously under unfavorable conditions that disrupt the integrity of the folded conformation. Thus, the chemical and physical environment of a protein, such as significant changes in pH...

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Scalable Flow Synthesis of Genetically Encodable Tetrazine Amino Acids.

Organic process research & development·2026
Same author

A Phosphorylation Switch Modulates Configurational Codes in the Oncofetal IGF2BP RNA Binding Paralogs.

bioRxiv : the preprint server for biology·2026
Same author

Efficient and Site-Specific Incorporation of 3-Nitro-Tyrosine Into Recombinant Proteins in <i>Escherichia coli</i>.

Bio-protocol·2026
Same author

Accessing intractable, phosphorylated intrinsically disordered proteins via a protease-cleavable inclusion body tag.

Protein science : a publication of the Protein Society·2026
Same author

Highly mutagenic continuous evolution in E. coli using a Φ29-based orthogonal replication system.

Nature biotechnology·2026
Same author

Endogenous Site-Specific Encoding of Trifluoromethyl-Bearing Phenylalanine and Tryptophan for in-Cell <sup>19</sup>F NMR.

Journal of the American Chemical Society·2026
Same journal

Gas-Responsive Metal-Organic Frameworks for Adaptive Thermal Energy Storage with Tunable Charge-Discharge Temperatures.

Journal of the American Chemical Society·2026
Same journal

Engineering a Thiamine-Dependent Benzoylformate Decarboxylase for Stereodivergent Radical C(sp<sup>3</sup>)-C(sp<sup>3</sup>) Bond Formation.

Journal of the American Chemical Society·2026
Same journal

Accelerated Directional Proton-Coupled Electron Transfer Enabled by Intrinsic Dipole Field in Biomimetic α-Helical Structure.

Journal of the American Chemical Society·2026
Same journal

Alternating Current-Driven Hydrogen Isotope Labeling of Aliphatic Amines Using 1,3-Propanedithiol as an Efficient Hydrogen Atom Transfer Reagent.

Journal of the American Chemical Society·2026
Same journal

Two-Dimensional van der Waals Polar Metal MoOBr<sub>2</sub>.

Journal of the American Chemical Society·2026
Same journal

Negatively Curved Chiral Bilayer Nanographene.

Journal of the American Chemical Society·2026
Ver todos los artículos relacionados
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Video Experimental Relacionado

Updated: Jul 6, 2026

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter
12:15

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Published on: May 29, 2019

El daño oxidativo de la proteína que codifica genéticamente el daño oxidativo.

Heinz Neumann1, Jennifer L Hazen, John Weinstein

  • 1Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, United Kingdom.

Journal of the American Chemical Society
|March 7, 2008
PubMed
Resumen
Este resumen es generado por máquina.

Los investigadores desarrollaron un método para insertar con precisión 3-nitrotirosina (3-NT) en proteínas, ayudando a la investigación de enfermedades. Esta técnica permite estudiar el impacto de 3-NT en la función de las proteínas en condiciones como la enfermedad de Alzheimer y la enfermedad de Parkinson.

Más Videos Relacionados

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
07:16

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation

Published on: June 21, 2021

Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors
09:33

Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors

Published on: February 7, 2018

Videos de Experimentos Relacionados

Last Updated: Jul 6, 2026

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter
12:15

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Published on: May 29, 2019

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
07:16

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation

Published on: June 21, 2021

Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors
09:33

Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors

Published on: February 7, 2018

Área de la Ciencia:

  • La bioquímica es la bioquímica.
  • Biología Molecular Biología Molecular
  • Ingeniería de proteínas Ingeniería de proteínas.

Sus antecedentes:

  • La modificación posttraducional que produce 3-nitrotirosina (3-NT) está relacionada con más de 50 enfermedades, incluidos los trastornos neurodegenerativos y el cáncer.
  • Los niveles elevados de 3-NT en los tejidos envejecidos predicen el riesgo de enfermedad, lo que pone de relieve la necesidad de estudiar su impacto funcional.

Objetivo del estudio:

  • Para diseñar un par aminoacil-tRNA sintetasa / tRNA para la incorporación cotranslacional de 3-NT en proteínas específicas del sitio.
  • Demostrar la utilidad de este sistema para investigar cómo las modificaciones específicas de 3-NT afectan la función de las proteínas en modelos de enfermedades.

Principales métodos:

  • Evolución y caracterización de un nuevo par aminoacil-tRNA sintetasa/tRNA.
  • Incorporación específica del sitio de 3-NT en proteínas durante la traducción.
  • Preparación de dismutasa de superóxido de manganeso homogéneamente nitrada (MnSOD) en un sitio relevante para la enfermedad.

Principales resultados:

  • Desarrollo exitoso de un sistema para la incorporación de 3-NT genéticamente codificada y específica del sitio.
  • Demostración de la utilidad del sistema mediante la producción de MnSOD. modificado homogéneamente con 3-NT.
  • Análisis de las consecuencias funcionales de la nitratación específica del sitio en la actividad de MnSOD.

Conclusiones:

  • El par aminoacil-tRNA sintetasa/tRNA diseñado permite un control preciso de la nitratación de las proteínas.
  • Este método proporciona una poderosa herramienta para estudiar el papel de 3-NT en varios estados de la enfermedad.
  • Facilita la investigación sobre las alteraciones de la función proteica en los trastornos neurológicos, la inflamación y el envejecimiento.