Search research articles

ACERCA DE JoVE

Visión General Liderazgo Blog Centro de Ayuda JoVE

AUTORES

Proceso de Publicación Consejo Editorial Alcance y Políticas Revisión por Pares Preguntas Frecuentes Enviar

BIBLIOTECARIOS

Testimonios Suscripciones Acceso Recursos Consejo Asesor de Bibliotecas Preguntas Frecuentes

INVESTIGACIÓN

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archivo

EDUCACIÓN

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Centro de Recursos para Profesores Sitio de Profesores

Términos y Condiciones de Uso

Política de Privacidad

Videos de Conceptos Relacionados

MAPK Signaling Cascades

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...

Interactions Between Signaling Pathways

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...

Amplifying Signals via Enzymatic Cascade

Amplifying Signals via Enzymatic Cascade

When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze the...

Assembly of Signaling Complexes

Assembly of Signaling Complexes

Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...

The JAK-STAT Signaling Pathway

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...

PI3K/mTOR/AKT Signaling Pathway

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a rapamycin-insensitive companion...

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por

Same author

Reimagining the Synthetic Biology DBTL Cycle with Machine Learning.

Methods in molecular biology (Clifton, N.J.)·2026

Same author

Opportunities for artificial intelligence and synthetic biology in designing living drug delivery systems.

Advanced drug delivery reviews·2026

Same author

Ultra-large targeted DNA integrations in primary human cells.

bioRxiv : the preprint server for biology·2026

Same author

DROP-CARs: Engineering Reversible, Drug-Controlled CAR T-cell Activity with a Clinically Approved Small Molecule.

Cancer research·2026

Same author

Spike-in probe-enhanced single-cell RNA-seq reveals post-infusion transcriptomic remodeling of "prime-and-kill" synNotch-CAR-T cells.

bioRxiv : the preprint server for biology·2026

Same author

Ultra-high-throughput mapping of genetic design space.

Nature·2026

Search research articles

Video Experimental Relacionado

Updated: Jul 6, 2026

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Published on: June 15, 2017

El uso de interacciones de andamio de ingeniería para remodelar la dinámica de señalización de la vía de la quinasa

Caleb J Bashor¹, Noah C Helman, Shude Yan

¹Department of Cellular and Molecular Pharmacology, University of California at San Francisco, 600 16th Street, San Francisco, CA 94158, USA.

Science (New York, N.Y.)

|March 15, 2008

Resumen

Las proteínas del andamio como Ste5 pueden ser diseñadas para controlar las vías de señalización celular. Esta investigación demuestra la reprogramación de las respuestas celulares para nuevas aplicaciones terapéuticas y biotecnológicas.

Más Videos Relacionados

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System

Published on: March 20, 2016

Assessing Cellular Target Engagement by SHP2 (PTPN11) Phosphatase Inhibitors

Assessing Cellular Target Engagement by SHP2 (PTPN11) Phosphatase Inhibitors

Published on: July 17, 2020

Videos de Experimentos Relacionados

Last Updated: Jul 6, 2026

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Published on: June 15, 2017

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System

Published on: March 20, 2016

Assessing Cellular Target Engagement by SHP2 (PTPN11) Phosphatase Inhibitors

Assessing Cellular Target Engagement by SHP2 (PTPN11) Phosphatase Inhibitors

Published on: July 17, 2020

Área de la Ciencia:

Biología Molecular Biología Molecular
Biología de Sistemas Biología de Sistemas.
La señalización celular.

Sus antecedentes:

Las proteínas del andamio son cruciales para ensamblar moléculas de señalización en complejos funcionales.
Estos andamios pueden actuar como centros de procesamiento de señales, integrando circuitos de retroalimentación para optimizar las respuestas celulares.
Comprender la función de la proteína del andamio es clave para manipular las vías de señalización.

Objetivo del estudio:

Para investigar el potencial de la proteína del andamio Ste5 como una plataforma para remodelar la salida de señalización celular.
Diseñar circuitos de retroalimentación sintéticos para modular la vía de la quinasa MAP de apareamiento de la levadura.
Explorar la aplicación de andamios de ingeniería en la reprogramación de las funciones celulares.

Principales métodos:

Construcción de circuitos sintéticos de retroalimentación positiva y negativa.
Regulación dinámica del reclutamiento del modulador de la vía a un sitio de unión artificial en el andamio Ste5.
Análisis de la salida de la vía de la quinasa MAP de apareamiento de levadura por ingeniería.

Principales resultados:

Demostrado que el andamio de la Etapa 5 puede ser reprogramado sistemáticamente para alterar la salida de la vía.
Los circuitos diseñados exhibieron diversos comportamientos, incluida la respuesta a la dosis ultrasensible, los tiempos de respuesta alterados y la adaptación sintonizable.
Creación exitosa de bucles de retroalimentación que controlaban dinámicamente la dinámica de las vías de señalización.

Conclusiones:

Los andamios de proteínas ofrecen una plataforma flexible para reprogramar la señalización celular.
Los andamios diseñados pueden utilizarse para crear nuevos comportamientos celulares.
Este enfoque tiene potencial para desarrollar nuevas aplicaciones terapéuticas y biotecnológicas.