Video Experimental Relacionado
Updated: Jul 6, 2026
13:00
Engineering Antiviral Agents via Surface Plasmon Resonance
Published on: June 14, 2022
Construcción en el virus de ligandos polivalentes de glicanos para los receptores de la superficie celular
Eiton Kaltgrad1, Mary K O'Reilly, Liang Liao
1Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
Journal of the American Chemical Society
|March 18, 2008
Resumen
Los investigadores crearon ligandos de carbohidratos complejos en las superficies de los virus. Estos ligandos se unen específicamente a los receptores objetivo, ofreciendo un nuevo método para estudiar las interacciones de los receptores agrupados.
Área de la Ciencia:
- Química de los hidratos de carbono.
- Virología Virología.
- La bioconjugación por bioconjugación.
Sus antecedentes:
- Los virus muestran estructuras superficiales bien definidas.
- Los glicanos en las superficies de los virus pueden ser modificados.
- El desarrollo dirigido de ligandos es crucial para estudiar las interacciones de la superficie celular.
Objetivo del estudio:
- Desarrollar un método para sintetizar ligandos de carbohidratos complejos utilizando partículas de virus.
- Para investigar las propiedades de unión de estos ligandos sintetizados.
- Explorar el potencial de este método para crear herramientas para estudiar receptores agrupados.
Principales métodos:
- Utilizando partículas de virus como andamios para las reacciones de la glucosiltransferasa.
- Sintetizando di- y trisacáridos directamente en el exterior del virus.
- Prueba de la afinidad de unión y la especificidad de las partículas de virus modificados a las cuentas y células recubiertas de receptores.
Principales resultados:
- Con éxito sintetizó glicanos complejos en las superficies de los virus.
- Se ha demostrado la unión estrecha y específica de los virus modificados a los receptores cognados.
- Se observaron efectos de unión polivalente, incluida la interrupción de las interacciones de la superficie celular in cis.
Conclusiones:
- Las partículas de virus sirven como plataformas efectivas para crear complejos ligandos de carbohidratos.
- Esta metodología ofrece un enfoque potente y conveniente para la preparación de ligandos para receptores agrupados.
- Los conjugados virus-glicano resultantes muestran potencial para aplicaciones en diagnósticos y terapias.
Más Videos Relacionados
Videos de Conceptos Relacionados
01:14Glycocalyx and its Functions
The glycocalyx is a carbohydrate-rich, fuzzy-appearing layer on the outer surface of the cell membrane. It is highly hydrophilic, because of this it attracts large amounts of water to the cell's surface. This aids the cell's interaction with the watery environment and also helps it to obtain substances dissolved in the water. It is also important for cell identification, self/non-self determination, and embryonic development and is used in cell-to-cell attachments to form tissues.
Components of...
Components of...
01:25Protein Glycosylation
Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...
Glycosylation occurs in...
01:24Oligosaccharide Assembly
Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
Multiple sugar molecules that may or may...
02:50Conjugated Proteins
Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
01:05Proteoglycans
Glycans, a class of complex heterogeneous molecules, can be covalently attached to proteins to form glycosylated proteins that regulate various physiological and pathological processes. Glycosylated proteins or glycoproteins comprise N-linked and O-linked oligosaccharides. O-glycosylation is the most common type of protein glycosylation. Here, glycans attach to the oxygen atom of the hydroxyl groups of Serine or Threonine residues. O-linked glycosylation occurs later in protein processing,...
01:31Immunoglobulin-like Cell Adhesion Molecules
Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...