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Identificación de un morfógeno epitelial derivado de los fibroblastos como factor de crecimiento hepatocítico

R Montesano¹, K Matsumoto, T Nakamura

¹Department of Morphology, University Medical Center, Geneva, Switzerland.

|November 29, 1991

Resumen

El factor de crecimiento hepatocitario (HGF) derivado de fibroblastos induce la tubulogénesis epitelial en las células renales caninas de Madin-Darby. Este hallazgo revela el papel del HGF en la señalización paracrina y la morfogénesis epitelial durante el desarrollo de los órganos.

Área de la Ciencia:

Biología celular Biología celular.
Biología del desarrollo Biología del desarrollo.
La bioquímica es la bioquímica.

Sus antecedentes:

Las células del riñón canino Madin-Darby (MDCK) forman quistes esféricos in vitro bajo condiciones estándar.
Estudios previos mostraron que los fibroblastos inducen la formación de túbulos ramificados en las células MDCK.

Objetivo del estudio:

Para identificar la molécula derivada del fibroblasto responsable de la inducción de la tubulogénesis epitelial.
Para investigar el papel del factor de crecimiento hepatocitario (HGF) en la morfogénesis epitelial.

Principales métodos:

Cultivo de células MDCK en geles de colágeno con o sin fibroblastos/medio acondicionado con fibroblastos (CM).
La adición de HGF exógeno a cultivos celulares de MDCK.
El uso de anticuerpos contra el HGF para neutralizar la actividad tubulogénica en el fibroblasto CM.

Principales resultados:

La adición exógena de HGF a las células MDCK indujo la formación de túbulos epiteliales.
Los anticuerpos contra el HGF bloquearon completamente la actividad tubulogénica del fibroblasto CM.
El HGF derivado de los fibroblastos es el mediador clave de la tubulogénesis epitelial.

Conclusiones:

El factor de crecimiento hepatocitario (HGF) actúa como mediador paracrino de la morfogénesis epitelial.
El HGF juega un papel importante en la formación de órganos parenquimales durante el desarrollo embrionario.
Este estudio identifica el HGF como un factor crítico en la formación del túbulo epitelial y la organogénesis.

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