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El complejo de cohesina promueve la terminación transcripcional entre genes convergentes en S. pombe
Monika Gullerova1, Nick J Proudfoot
1Sir William Dunn School of Pathology, University of Oxford, South Parks Rd, Oxford OX1 3RE, UK.
Cell
|March 25, 2008
Resumen
El ARN de doble hebra en S. pombe desencadena la interferencia del ARN y la formación de heterocromatina, reclutando a la cohesina para bloquear la producción adicional de dsRNA y regular la terminación de la transcripción.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- La epigenética es la epigenética.
- Regulación genética Reglamento genético.
Sus antecedentes:
- Los genes convergentes en S. pombe producen transcripciones superpuestas durante la fase G1.
- El ARN de doble hebra (dsRNA) está implicado en la regulación génica.
Objetivo del estudio:
- Investigar el papel del dsRNA en la formación de la heterocromatina y la terminación de la transcripción.
- Para aclarar la función de la cohesina en el contexto de los cambios epigenéticos inducidos por dsRNA.
Principales métodos:
- Análisis de la transcripción en S. pombe.
- Análisis de la vía de interferencia de ARN (RNAi) utilizando Dicer y RITS.
- Inmunoprecipitación de la cromatina para detectar las modificaciones de las histonas (H3K9me3) y la asociación de proteínas (Swi6, cohesina).
Principales resultados:
- El dsRNA induce la heterocromatina localizada y dependiente del ARN con H3K9me3 y Swi6.6.
- La cohesina se recluta en estas regiones heterocromáticas a través de Swi6.
- La cohesina se concentra en regiones intergenéticas de genes convergentes en G2, promoviendo la terminación de la transcripción y bloqueando la síntesis de dsRNA.
- La cohesina se libera en la mitosis, iniciando un nuevo ciclo.
Conclusiones:
- La cohesina juega un papel novedoso en la regulación de la terminación transcripcional.
- La formación de dsRNA, heterocromatina y el posterior reclutamiento de cohesión es un mecanismo generalizado para la terminación regulada de la transcripción.
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