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La cinasa CTD está asociada con el factor b de iniciación de la ARN polimerasa II de la levadura
1Department of Cell Biology, Stanford University School of Medicine, California 94305.
Cell
|December 20, 1991
Resumen
El factor de iniciación general b de la levadura posee actividad quinasa que fosforila la ARN polimerasa II.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- La bioquímica es la bioquímica.
- Factores de transcripción Factores de transcripción
Sus antecedentes:
- La fosforilación de la ARN polimerasa II del dominio C-terminal (DTC) es crucial para la transcripción.
- Los factores generales de iniciación de la transcripción juegan un papel esencial en la regulación de la expresión génica.
Objetivo del estudio:
- Para identificar y caracterizar la actividad de la quinasa asociada con el factor de iniciación general de la levadura b.
- Para investigar el papel de esta quinasa en la iniciación de la transcripción.
Principales métodos:
- Cofraccionamiento basado en el tamaño y la carga.
- Inactivación por calor leve.
- Pruebas de kinasa. ensayos de kinasa.
- Pruebas de la ATPasa dependiente del ADN.
Principales resultados:
- Se encontró que una actividad de quinasa específica para la ARN polimerasa II CTD estaba asociada con el factor de iniciación general de la levadura b.
- Esta quinasa fosforila el CTD en múltiples sitios.
- El factor b también exhibe actividad de ATPasa dependiente del ADN.
- Ambas actividades comparten los requisitos de nucleótidos con la iniciación de la transcripción.
Conclusiones:
- La fosforilación de la CTD por el factor b está involucrada en la iniciación de la transcripción.
- El factor b posee actividades catalíticas esenciales para el proceso de iniciación de la transcripción.
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