Video Experimental Relacionado
Updated: Jun 27, 2026
22:27
Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.
Published on: May 7, 2010
La estructura 3D del locus de la cadena pesada de la inmunoglobulina: implicaciones para las interacciones genómicas
Suchit Jhunjhunwala1, Menno C van Zelm, Mandy M Peak
1Division of Biological Sciences, 0377, University of California, San Diego, La Jolla, CA 92093, USA.
Cell
|April 22, 2008
Resumen
El locus de la cadena pesada de inmunoglobulina (Igh) forma compartimentos con racimos en bucle. Durante el desarrollo de las células B, las regiones V (H) se fusionan con los elementos D (H) -J (H), facilitando las interacciones genómicas.
Área de la Ciencia:
- La genómica es la genómica.
- Inmunología Inmunología.
- Biología de la cromatina Biología de la cromatina
Sus antecedentes:
- El locus de la cadena pesada de inmunoglobulinas (Igh) contiene numerosos elementos de codificación (V, H, D, H, J, H, C, H).
- La organización tridimensional (3D) del locus Igh sigue siendo en gran medida no caracterizada.
Objetivo del estudio:
- Para investigar la estructura 3D y la topografía del locus Igh.
- Para entender las disposiciones de la cromatina dentro del locus Igh.
Principales métodos:
- Las distribuciones de distancias espaciales se midieron entre 12 marcadores genómicos que abarcan el locus Igh.
- Se utilizaron simulaciones por computadora para modelar arreglos alternativos de cromatina.
- Mediciones de trilateración y de ángulo de punto triple determinaron posiciones 3D relativas de los elementos Igh.
Principales resultados:
- El locus Igh está organizado en compartimentos distintos que comprenden grupos de bucles conectados por enlaces.
- Se observaron compartimentación y cambios conformacionales significativos que involucraban elementos V (H) y D (H) -J (H) durante el desarrollo temprano de las células B.
- En las células pro-B, las regiones V (H) (2 Mbp) se yuxtaponen con elementos D (H), lo que permite frecuentes interacciones genómicas de largo alcance.
Conclusiones:
- El locus Igh exhibe una estructura 3D compartimentada con cromatina en bucle.
- La dinámica conformal del locus Igh es crucial para la accesibilidad del gen V (H) y el desarrollo de las células B.
Videos de Conceptos Relacionados
01:10Antibody Structure
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
01:10Antibody Structure
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
01:49Conserved Binding Sites
Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally analyses the...
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally analyses the...
02:35Chromatin Position Affects Gene Expression
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area.
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the timing and level of...
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the timing and level of...
01:28Diversity of Antigen Receptors
Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
01:25Antibody Structure and Classes
Antibodies, also known as immunoglobulins, are produced by B cells in response to foreign substances, such as bacteria and viruses. These proteins are critical for recognizing and neutralizing these substances, protecting the body from potential harm.
The basic structure of an antibody consists of four protein chains: two identical heavy chains and two identical light chains. These chains are held together by disulfide bonds and other non-covalent interactions, forming a Y-shaped structure.
The basic structure of an antibody consists of four protein chains: two identical heavy chains and two identical light chains. These chains are held together by disulfide bonds and other non-covalent interactions, forming a Y-shaped structure.