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Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins
Published on: January 17, 2014
El virus Vaccinia utiliza la macropinocitosis y la imitación apoptótica para entrar en las células huésped
1ETH Zurich, Institute of Biochemistry, Schafmattstrasse 18, ETH Hönggerberg HPM E6.3 Zurich, Switzerland.
Science (New York, N.Y.)
|April 26, 2008
Resumen
El virus de Vaccinia entra en las células huésped mediante macropinocitosis dependiente del pH, que involucra filopodia y blebs de membrana. Este proceso imita la apoptosis mediante la utilización de fosfatidilserina expuesta en la membrana viral para la entrada.
Área de la Ciencia:
- Virología Virología.
- Biología celular Biología celular.
- Biología Molecular Biología Molecular
Sus antecedentes:
- Los virus utilizan diversos mecanismos para entrar en la célula huésped.
- Comprender las vías de entrada viral es crucial para desarrollar estrategias antivirales.
Objetivo del estudio:
- Para dilucidar el mecanismo de entrada del virus de la vacuna en las células huésped.
- Para investigar el papel de las vías celulares específicas y los componentes virales en la infección por el virus de la vacuna.
Principales métodos:
- Imágenes de células vivas de las partículas del virus de la vacuna con etiqueta fluorescente.
- Análisis de la activación de la quinasa 1 activada por p21 (PAK1).
- Caracterización del proceso endocítico y su dependencia de la fosfatidilserina.
Principales resultados:
- El virus Vaccinia utiliza filopodia para llegar al cuerpo celular e induce las manchas de la membrana para la internalización.
- El proceso de entrada es dependiente del pH y exhibe características de la macropinocitosis.
- La quinasa 1 activada por p21 (PAK1) se activa durante la entrada viral.
- La fosfatidilserina expuesta en la membrana viral es esencial para la inducción de bleb, la endocitosis y la infección.
Conclusiones:
- El virus Vaccinia emplea la imitación apoptótica, utilizando fosfatidilserina expuesta para facilitar la entrada a través de la macropinocitosis.
- Los hallazgos revelan una nueva estrategia de entrada viral que involucra el blebbing de membrana y el tráfico filopodial.
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