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La regulación alostérica de las histidina quinasas por su regulador de respuesta cognado determina el destino celular
Ralf Paul1, Tina Jaeger, Sören Abel
1Biozentrum, University of Basel, Klingelbergstrasse 70, 4056 Basel, Switzerland.
Cell
|May 6, 2008
Resumen
La proteína DivK en Caulobacter crescentus regula el destino de las células bacterianas. Actúa como un regulador alostérico, controlando la fosforilación y la localización a través de circuitos de retroalimentación dentro del sistema de dos componentes.
Área de la Ciencia:
- Microbiología Microbiología.
- Fisiología bacteriana fisiología bacteriana.
- Biología celular Biología celular.
Sus antecedentes:
- Los sistemas de dos componentes son cruciales para el crecimiento bacteriano y la fisiología.
- Las vías de transducción de señales gobiernan la determinación del destino de las células bacterianas.
- Caulobacter crescentus utiliza la organización espacial para la diferenciación celular.
Objetivo del estudio:
- Para investigar la plasticidad y la interconexión de las vías de transducción de señales en Caulobacter crescentus.
- Para aclarar el papel de la proteína DivK en la regulación del destino de las células bacterianas.
- Comprender los mecanismos de retroalimentación dentro de la red de fosforilación de dos componentes.
Principales métodos:
- Análisis de las actividades antagónicas entre la PleC fosfatasa y la DivJ quinasa.
- Investigando la regulación alostérica por DivK en PleC y DivJ.
- Estudiando el programa morfogenético cíclico di-GMP dependiente.
Principales resultados:
- DivK actúa como un regulador alostérico, cambiando el estado de PleC de fosfatasa a autoquinasa.
- DivK media en un programa morfogenético cíclico di-GMP dependiente.
- DivK estimula su propia fosforilación y localización polar a través de la activación alostérica de DivJ.
Conclusiones:
- DivK es un efector central en un circuito integrado que controla la asimetría bacteriana y el destino celular.
- Los bucles de retroalimentación organizados espacialmente son esenciales para la determinación del destino celular en C. crescentus.
- Los reguladores de respuesta de dominio único facilitan la retransmisión y la retroalimentación en redes de dos componentes.
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