Video Experimental Relacionado

Updated: Apr 29, 2026

Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4

Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4

Published on: December 31, 2013

Pirt, una proteína que se une a los fosfoinosítidos, funciona como una subunidad reguladora del TRPV1

Andrew Y Kim¹, Zongxiang Tang, Qin Liu

¹The Solomon H. Snyder Department of Neuroscience, Center for Sensory Biology, School of Medicine, Johns Hopkins University, Baltimore, MD 21205, USA.

|May 6, 2008

Resumen

Una proteína recientemente descubierta, Pirt, actúa como un regulador crucial para el canal del receptor transitorio potencial de vanilloide 1 (TRPV1). Pirt mejora la respuesta de TRPV1 al calor y la capsaicina, lo que afecta la sensación de dolor.

Área de la Ciencia:

Biología molecular La biología molecular.
La neurociencia es la neurociencia.
Investigaciones sobre el dolor.

Sus antecedentes:

El Vanilloide Potencial del Receptor Transiente 1 (TRPV1) es un sensor clave para el calor nocivo y la capsaicina, involucrado en la percepción del dolor.
Se sabe que la actividad del canal TRPV1 está modulada por varios mecanismos.
Los agentes moleculares precisos que regulan la función de TRPV1 siguen siendo un área activa de investigación.

Objetivo del estudio:

Para identificar nuevos reguladores de la actividad del canal TRPV1.
Para aclarar el papel de la proteína de membrana Pirt en la función de TRPV1 y la nocicepción.

Principales métodos:

Utilizó ratones Pirt nulos para evaluar el impacto fisiológico de la deficiencia de Pirt en la termo-sensación y la quimio-sensación.
Se realizaron grabaciones electrofisiológicas en las neuronas del ganglio de la raíz dorsal (DRG) de ratones de tipo salvaje y ratones con deficiencia de Pirt.
Investigó la interacción entre Pirt y TRPV1 a través de sistemas de expresión heterólogos y ensayos de unión bioquímica (terminal C, fosfoinosítidos como PIP2).

Principales resultados:

Los ratones Pirt null mostraron respuestas significativamente reducidas al calor nocivo y a la capsaicina.
Las neuronas DRG de ratones con deficiencia de Pirt mostraron corrientes atenuadas provocadas por el calor y la capsaicina.
Coexpresión de Pirt con corrientes mediadas por TRPV1 potenciadas por TRPV1; el extremo C de Pirt une TRPV1 y PIP2, una unión esencial para la función reguladora de Pirt.

Conclusiones:

Pirt se identifica como un nuevo regulador positivo de la actividad del canal TRPV1.
Pirt, a través de su interacción con TRPV1 y PIP2, juega un papel crítico en la modulación de la señalización nociceptiva.
Pirt es un componente clave del complejo TRPV1, esencial para su función adecuada en la detección de estímulos nocivos.

Más Videos Relacionados

Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation

Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation

Published on: January 6, 2016

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

Published on: July 26, 2019

Videos de Conceptos Relacionados

Thermosensation

Thermosensation

Peripheral thermosensation is the perception of external temperature. A change in temperature (on the surface of the skin and other tissues) is detected by a family of temperature-sensitive ion channels called Transient Receptor Potential, or TRP, receptors. These receptors are located on free nerve endings. Those detecting cold temperatures are closer to the surface of the skin than the nerve endings detecting warmth. These thermoTRP channels, while temperature selective, have relatively...

Protein Kinases and Phosphatases

Protein Kinases and Phosphatases

Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...

Phosphoinositides and PIPs

Phosphoinositides and PIPs

Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...

Activation and Inactivation of G Proteins

Activation and Inactivation of G Proteins

Heterotrimeric G proteins are guanine nucleotide-binding proteins. As the name suggests, heterotrimeric G proteins are composed of three subunits: alpha, beta, and gamma. They remain GDP-bound or GTP-bound inside the cells and switch between inactive/active states. The Gα subunit possesses the nucleotide-binding pocket that binds guanine nucleotides and switches between GDP or GTP-bound states. In contrast, the Gꞵ and Gγ subunits are always bound together with high...

G-Protein Gated Ion Channels

G-Protein Gated Ion Channels

GPCRs are primarily responsible for our sense of smell, taste, and vision. The binding of a sensory stimulus activates GPCR to stimulate effector proteins, many of which are ion channels in the sensory organs. GPCRs modulate the opening and closing of the target ion channels either directly by binding them, or by releasing second messengers that activate these channels. As ions move across the membrane, the membrane potential is altered, which induces an appropriate response.
Sensory...

IP3/DAG Signaling Pathway

IP3/DAG Signaling Pathway

Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and...