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Control global de la transcripción del ciclo celular por CDK acoplados y osciladores de red
David A Orlando1, Charles Y Lin, Allister Bernard
1Department of Biology, Duke University, Durham, North Carolina 27708, USA.
Nature
|May 9, 2008
Resumen
La expresión génica de la levadura en ciernes está programada para la división celular. Las cinasas dependientes de ciclina (CDK) influyen en esto, pero una red de factores de transcripción también impulsa la transcripción periódica de genes de forma independiente.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Genética La genética.
- Biología celular Biología celular.
Sus antecedentes:
- El tiempo de expresión génica es crucial para la progresión del ciclo celular en Saccharomyces cerevisiae.
- Las redes de factores de transcripción y las cinasas dependientes de la ciclina (CDK) están implicadas en la regulación de la transcripción dependiente del ciclo celular.
- Estudios previos sugirieron que la actividad de CDK gobierna principalmente la transcripción periódica de genes.
Objetivo del estudio:
- Investigar el papel de los complejos ciclina-CDK en el control del programa global de transcripción del ciclo celular.
- Determinar si las CDK son las únicas responsables de establecer el programa de transcripción periódica durante el ciclo celular.
Principales métodos:
- Se analizaron las dinámicas de transcripción de todo el genoma en mutantes de levadura en ciernes que carecían de ciclinas S-fase y mitóticas.
- La progresión del ciclo celular fue monitoreada en estos mutantes con deficiencia de ciclina.
Principales resultados:
- Una porción significativa de los genes expresados periódicamente mostraron una expresión aberrante en los mutantes de la ciclina.
- A pesar de la detención del ciclo celular en la frontera G1/S, aproximadamente el 70% de los genes periódicos mantuvieron su expresión periódica programada.
- La actividad ciclina-CDK no es el único determinante del programa de transcripción periódica global.
Conclusiones:
- Las cinasas dependientes de la ciclina juegan un papel en la regulación de la transcripción del ciclo celular, pero no controlan exclusivamente todo el programa.
- La transcripción periódica de genes parece ser una propiedad emergente de una red de factores de transcripción.
- Esta red de factores de transcripción puede funcionar como un oscilador del ciclo celular, operando independientemente o en conjunto con el oscilador CDK.
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