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En la superficie celular, se requieren moléculas similares a la heparina para la unión del factor de crecimiento
A Yayon1, M Klagsbrun, J D Esko
1Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel.
Cell
|February 22, 1991
Resumen
Los sitios de unión al heparina de baja afinidad son moléculas accesorias esenciales para la unión al receptor del factor de crecimiento de los fibroblastos básicos (bFGF) de alta afinidad. Estos sitios regulan las interacciones de los factores de crecimiento, revelando un nuevo mecanismo en la señalización celular.
Área de la Ciencia:
- Biología celular Biología celular.
- La bioquímica es la bioquímica.
- Biología Molecular Biología Molecular
Sus antecedentes:
- El factor básico de crecimiento de los fibroblastos (bFGF) es crucial para el crecimiento y la diferenciación celular.
- La interacción de bFGF con su receptor es crítica para la transducción de señales.
- El papel de los sitios de unión de baja afinidad en esta interacción aún no se ha aclarado completamente.
Objetivo del estudio:
- Para investigar la función de la baja afinidad, heparina-como sitios de unión para bFGF.
- Para determinar si estos sitios son necesarios para la unión de bFGF de alta afinidad.
- Comprender el papel regulador de estos sitios en las interacciones factor-receptor de crecimiento.
Principales métodos:
- Se utilizaron células de ovario de hámster chino (CHO) con mutaciones en el metabolismo del glucosaminoglicano.
- Células mutantes trasfectadas con un ADNc del receptor FGF de ratón clonado.
- Se evaluó la unión al bFGF en presencia y ausencia de heparina libre y sulfato de heparán.
Principales resultados:
- Las células CHO deficientes en sulfato de heparano que expresan los receptores de FGF no pudieron unirse al bFGF.
- Heparina libre y sulfato de heparan reconstituido de baja afinidad bFGF.
- Este receptor de baja afinidad era necesario para la unión de bFGF de alta afinidad.
Conclusiones:
- Los receptores similares a la heparina de baja afinidad actúan como moléculas accesorias para la unión del bFGF.
- Estos receptores son obligatorios para la interacción de alta afinidad de bFGF con su receptor.
- Esto pone de relieve un nuevo mecanismo regulador para la señalización del factor de crecimiento-receptor que involucra moléculas similares a la heparina.
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