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El factor ectodérmico restringe la diferenciación del mesodermo al inhibir el p53

Noriaki Sasai¹, Rieko Yakura, Daisuke Kamiya

¹Organogenesis and Neurogenesis Group, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan. norisa@cdb.riken.jp

|May 31, 2008

Resumen

Una nueva proteína de dedo de zinc, XFDL156, actúa como un factor ectodérmico que suprime la diferenciación mesodérmica durante la gastrulación de anfibios al inhibir la actividad de p53.

Área de la Ciencia:

Biología del desarrollo Biología del desarrollo.
Biología molecular y celular Biología molecular y celular

Sus antecedentes:

El desarrollo embrionario se basa en la especificación precisa de la capa germinal.
La formación del mesodermo involucra la señalización de TGF-beta y p53, pero los factores cigóticos clave siguen sin identificarse.

Objetivo del estudio:

Identificar nuevos factores zigóticos que regulan la especificación del mesodermo durante la gastrulación de anfibios.
Para aclarar el papel de XFDL156 en la diferenciación ectodérmica y mesodérmica.

Principales métodos:

Sobreexpresión y agotamiento de la proteína del dedo de zinc XFDL156 en embriones de anfibios.
Análisis de los marcadores mesodérmicos y la inducción del gen diana p53.
Investigación de las interacciones proteína-proteína entre XFDL156 y p53.

Principales resultados:

Se identificó XFDL156, un factor cigótico ectodérmico.
La sobreexpresión de XFDL156 inhibió los marcadores de diferenciación mesodérmica.
El agotamiento de XFDL156 condujo a la diferenciación mesodérmica ectópica en el ectodermo.
XFDL156 interactúa con p53, inhibiendo su activación del gen diana y la diferenciación mesodérmica.

Conclusiones:

XFDL156 suprime activamente la diferenciación mesodérmica en el ectodermo.
Esta supresión está mediada por el control de la actividad de p53 y su capacidad de respuesta espacio-temporal.

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