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Extraction of Cofactor F420 for Analysis of Polyglutamate Tail Length from Methanogenic Pure Cultures and Environmental Samples
Published on: October 14, 2021
Estructura de una variante F430 de arqueas asociada con la oxidación anaeróbica del metano
Stefan Mayr1, Christopher Latkoczy, Martin Krüger
1Laboratory of Organic Chemistry, ETH Zurich, Wolfgang-Pauli-Strasse 10, 8093 Zurich, Switzerland.
Journal of the American Chemical Society
|July 23, 2008
Resumen
Los investigadores descubrieron un nuevo cofactor que contiene azufre, (17(2) S)-17(2)-metiltio-F430, en las alfombras microbianas del Mar Negro. Este cofactor es crucial para la oxidación anaeróbica del metano (AOM), pero puede formar un artefacto durante el aislamiento de la muestra.
Área de la Ciencia:
- Biogeoquímica La biogeoquímica es la bioquímica.
- Microbiología Microbiología.
- La bioquímica es la bioquímica.
Sus antecedentes:
- Las alfombras microbianas en el Mar Negro realizan la oxidación anaeróbica del metano (AOM) utilizando sulfato.
- Estas alfombras contienen proteínas similares a la metil-coenzima M reductasa, lo que sugiere un papel en el metabolismo del metano.
Objetivo del estudio:
- Identificar y caracterizar los cofactores que contienen níquel implicados en la MMA en las alfombras microbianas del Mar Negro.
- Determinar la estructura y la configuración de nuevos cofactores e investigar su origen.
Principales métodos:
- Espectrometría de masas (ICP-MS) para determinar la masa del cofactor y la composición elemental.
- Cromatografía líquida de alto rendimiento (HPLC) para la purificación de cofactores y derivados.
- Espectroscopia de resonancia magnética nuclear (RMN) para elucidación estructural.
- Catálisis ácida para estudiar la estabilidad del cofactor y la formación de artefactos.
Principales resultados:
- Se identificaron dos cofactores que contienen níquel: la coenzima F430 (m/z = 905) y una nueva especie (m/z = 951).
- El nuevo cofactor fue identificado como (17(2) S)-17(2)-metiltio-F430 pentametil éster, que contiene un átomo de azufre adicional.
- El isómero (17(2) S) es la forma natural, mientras que el más estable (17(2) R) es un artefacto formado bajo condiciones de aislamiento ácido.
Conclusiones:
- Un nuevo cofactor que contiene azufre, (17(2) S)-17(2)-metiltio-F430, está involucrado en la oxidación anaeróbica del metano en entornos marinos.
- Es necesario un control cuidadoso de las condiciones de aislamiento para evitar la formación de artefactos y estudiar con precisión las estructuras de cofactores naturales.
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